Related topics Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.430 kDa and an isoelectric point of 4.7. Bovine serum albumin is widely used in biochemical experiments, for example, as a Blocking agent in western blot. Uses 1, used in biochemical research, genetic engineering and medical research; 2, used as medical health food and condiment; 3, maintained osmotic pressure, pH buffering and carrier function; 4, contributed to the stability and activity of Taq enzyme in PCR system, which can improve the efficiency of PCR. The production method uses bovine blood as raw material to separate serum, and then uses ammonium sulfate to fractionate and precipitate it, and then it is refined by octanoic acid treatment. Storage method: Add 100 ml of water per10 g, or dissolve with PBS, depending on the application. And then sub-package into small branches. If preservative is not used, it can be stored in a constant temperature cabinet at minus 20 or 30 degrees Celsius, and if preservative (such as 0. 1% sodium azide) is used, it can be stored in an environment at minus 4 degrees Celsius. Generally, it can be stored for months without deterioration. Preservatives may affect the effect of bovine serum albumin and should be used with caution. Structure and functional components Structure The simple protein in bovine serum is the main component of blood (38g/ 100ml) with a molecular weight of 68kD. Isoelectric point 4.8. Nitrogen content 16%, sugar content 0.08%. It only contains hexose and hexosamine, and the fat content is only 0.2%. Albumin is composed of 58 1 amino acid residues, of which 35 cysteines are composed of 17 disulfide bonds, and there is a free sulfhydryl group at the 34th position of the peptide chain. Albumin can be combined with many cations, anions and other small molecules. Albumin in blood mainly plays the role of maintaining osmotic pressure, PH buffering, carrier and nutrition. In serum-free culture of animal cells, adding albumin can play a physiological and mechanical protection and carrier role. Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.430 kDa and an isoelectric point of 4.7. Bovine serum albumin is widely used in biochemical experiments, for example, as a Blocking agent in western blot.