2. It is advisable to use sawdust and rice bran as culture materials, and it is best to accumulate them for more than one year;
3. The surface of the culture material should be dense, and the culture material in each bottle should be uniform, elastic and consistent, and the bottle cap should be sterilized immediately after sealing;
4. The strain is required to cover the surface of the culture medium, which can make the mycelium grow evenly and effectively prevent the pollution of miscellaneous bacteria;
5. Transfer the inoculated vials to the culture room in time, and control the temperature, humidity and ventilation. After 20 ~ 25 days, hyphae can grow full;
6. Remove the old seed blocks and skins by scraper or hand, and scrape to make the fruiting bodies develop orderly from the surface of the culture medium;
7. After catching the bacteria, it is necessary to accelerate germination in time and give enough low-temperature stimulation to promote the formation of primordia;
8. Uniform cultivation is the transitional stage of inhibition treatment, and the air environment should be close to the natural state to promote the differentiation and branching of mushroom buds in low temperature environment.