1. Human diploid cell vaccine (HDCV): It is the most ideal cell culture vaccine by inoculating human embryonic lung fibroblasts with Pitman-Moore virus strain. The advantages of diploid cell vaccine are that the antibody appears quickly after inoculation, and almost reaches the positive conversion of 100% in 4 days. The antibody level is high and lasts for a long time, and neutralizing antibodies still exist in 5 years. If injected 1 time after 5 years, a good antibody reaction can be stimulated. However, because human diploid cells can only be cultured in small bottles, the number of cultures is limited, and the cost of large-scale industrial production is too high and difficult to popularize.
2. hamster kidney cell vaccine (PHKCV): primary hamster kidney cell culture vaccine, 197 1 used in Canada and the former Soviet Union. In China, 198 1, goat brain vaccine was replaced by PHKCV. The strain used for vaccine production in China is Beijing strain, which is prepared from primary hamster kidney cells and guinea pig brain. After long-term laboratory and epidemiological observation, it has been proved to be a safe and effective vaccine.
3. Purified Vero cell rabies vaccine (PVPV): Vero cell is an aneuploid cell, which has been derived from rhesus monkey kidney cell culture and is suitable for mass culture of microcarriers. Rabies virus PM 1503-3M proliferated in Vero cells, and the virus was harvested from the cell supernatant. The virus was inactivated by adding β -propiolactone after concentration, and the final product was obtained by adding albumin after purification. In addition, there are purified duck embryo rabies vaccine (PDEV), refined chicken embryo cell rabies vaccine (PCECV) and primary fetal bovine kidney cell rabies vaccine (FBKCV).
The Shanghai Center for Disease Control and Prevention has repeatedly demonstrated the potency, immune effect and safety of the vaccine, and has now approved two kinds of rabies vaccines for people in Shanghai, namely Wilport rabies vaccine produced by Pasteur Company of France and Vero cell produced by Changchun Changsheng Industrial Co., Ltd. ..