2, peeled potato slices 1000 ml boiling water for 30 minutes, after cooking, filter with gauze to get juice, pay attention to the water must be enough 1000 ml, not enough can be filled with boiling water, pour the potato juice into a container filled with other ingredients, while stirring, stirring evenly.
3. Suck the mixed liquid into the test tube, which is about one third of the test tube. Plug the test tube mouth with cotton, wrap five or six packed test tubes with kraft paper and tie them with ropes. Do not cover the lower part for future operation and observation. Put the tied test tubes into a high-pressure box for high-pressure disinfection.
4, pressure cooker temperature control in 120 degrees, disinfection for 30 minutes, take out the test tube and put it sideways while it is hot, so that the liquid in the test tube has an inclined plane after solidification, which is beneficial to the growth of mycelium.
5. Take fresh Pleurotus ostreatus, cut off a small piece in the middle with a small knife and put it in a test tube for culture. The whole process should be operated in a sterile environment, and the whole instrument should be disinfected with 75% alcohol.
6. Put it in an incubator, keep the temperature at 25 degrees, and the mother strain of Pleurotus ostreatus will be successfully cultivated in about ten days.