(2) Bud grafting propagation

(3) The growth and reproduction of yeast itself

(4) Temperature is an important condition for yeast growth and fermentation, and about 20℃ is most suitable for yeast propagation, so it is necessary to control the temperature within its optimum temperature range; Acetobacter belongs to psychrophilic bacteria, and the optimum growth temperature is 30-35℃, so the temperature should be controlled at 30-35℃.

(5)C 12h 22o 1 1+H2O 7C 6h 12o 6； C 6 H 12 O 6 2C 2 H 5 OH+2CO 2

(6) Acetobacter is an aerobic bacterium, which needs the participation of oxygen when converting alcohol into acetic acid, so it is necessary to aerate the fermentation broth in time.