The gel is made by dissolving agarose, an almost sulfate-free agar with polysaccharides as its main component, in hot water, pouring it into a glass, and cooling it. After melting at 37 ° C can be maintained in a liquid state for several hours, 30 ° C when solidified into a gel. That is to complete the configuration of agarose gel.

Made of small particles for gel filtration, suitable for sephadex can not be graded separation of large molecules of gel filtration, if the use of 5% or less concentration of the gel, but also capable of graded separation of cell particles, viruses, etc.. It is also used as a support for immunoelectrophoresis or in-gel sedimentation reaction instead of agar because of its small adsorption.

Expanded Information:

Agarose is divided into general agarose, which can be used as the support for immunoelectrophoresis or in-gel sedimentation. p>Agarose is divided into general agarose and low melting point agarose with reduced melting point after chemical modification, the melting point of agarose is between 62?65°C. It is mostly used for in-situ digestion of chromosomal DNA within the gel and recovery of DNA fragments. Agarose gels are often used for the separation of large proteins and DNA due to their large pore size.

Agar consists of agarose and agar pectin:

As a gelling agent, agarose is a non-ionic polysaccharide without sulfate ester, which is a component of the gel, and its macromolecular chain links with 1,3 glycosidic bonds alternately connected with β-D-galactose residues and 3,6-endo-lactose residues. Agar pectin, on the other hand, is the non-gelatinized portion, a complex polysaccharide with sulfate, glucuronic acid and pyruvate aldehyde, and the portion that is sought to be removed in commercial extraction.

Commercial agar generally carries 2% to 7% sulfate, 0% to 3% pyruvate aldehyde and 1% to 3% methylethyl. In the industrial agar Color from white to slightly yellow, with a gelatinous feel, odorless or with a slight characteristic odor, agar is insoluble in cold water, easily soluble in boiling water.

The agar is a natural high molecular polysaccharide substance which is refined and purified by scientific method, using high quality natural seaweeds such as rockweed, Gracilaria, purslane and so on as raw materials.

Agar is hydrophilic colloid, divided into strip and powder, insoluble in cold water, soluble in hot water. Agar has a unique importance in the industry, even if the concentration of agar is as low as 1%, it can still form a stable gel, which is a necessary raw material for the food industry, chemical industry and medical research.

Baidu Encyclopedia - Agarose Gel

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