Identification method of Rhizoma Anemarrhenae: (1) Take 2g of this product powder, add ethanol 10ml, shake for 20min, absorb supernatant 1ml, evaporate to dryness, and add sulfuric acid 1 drop to the residue, which turns yellow first, then red, purple and finally brown.
(2) Take 2g of this product powder, add 20ml of ethanol, heat and reflux for 40 minutes, take 65438 00 ml of supernatant, add 65438 00 ml of hydrochloric acid, heat and reflux for 65438 00 hours, then concentrate to about 5ml, add 65438 00 ml of water, shake and extract with 20ml of benzene, evaporate the extract, and dissolve the residue with 2ml of benzene as the test sample. In addition, a reference sample of Smilax sapogenin was added with benzene to prepare a solution containing 5mg per 1ml as a reference solution. According to the thin-layer chromatography test (Appendix ⅵ b), absorb 7μl of each of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, unfold them with benzene-acetone (9: 1) as developing agent, take them out, dry them in the air, and spray the mixed solution (0 7→ 10/0) of 8% vanillin absolute ethanol solution and sulfuric acid solution. In the chromatogram of the test sample, spots with the same color appear in the position corresponding to the chromatogram of the control sample.
The wholesale price of Anemarrhena asphodeloides is generally about one kilogram in 20 yuan, but the price also depends on the product quality. Please note that Chinese herbal medicines are bought for treating diseases. Please pay attention when buying them.