[Abstract] The invention belongs to the field of cellulase hydrolysis, and discloses a method for converting agricultural and forestry wastes into fermentable sugars. The method comprises the following steps: carrying out steam pretreatment and water washing on the crushed agricultural and forestry wastes, and then carrying out enzymolysis saccharification, wherein an active agent is used in the enzymolysis saccharification process. Compared with the previous methods, the invention avoids the ineffective adsorption of lignin to cellulase by adding the active agent, improves the effective concentration of cellulase, and solves the problem of large enzyme consumption in the traditional hydrolysis process. The invention has the advantages of less enzyme consumption, simple process, high sugar conversion rate and the like.
087 is a recombinant host suitable for simultaneous saccharification and fermentation.
[Abstract] The present invention provides a recombinant host cell, which comprises at least one heterologous polynucleotide encoding a polysaccharide enzyme, and the polynucleotide is under the transcriptional control of an alternative promoter capable of enhancing the expression of the polysaccharide enzyme. In addition, the present invention also provides a gene encoding a secretory protein to the above-mentioned host, so as to promote the secretion of the expressed polysaccharide enzyme. The preferred host of the present invention is ethanol-producing and capable of simultaneous saccharification and fermentation, resulting in the production of ethanol from complex cellulose substrates.
04 1 method for producing industrial enzymes with waste molasses as main fermentation raw material
[Abstract] The invention belongs to the technical field of industrial biology and fermentation engineering. Includes: 1) preparing culture medium solution with waste molasses as main carbon source; 2) inoculating enzyme-producing strains into culture medium solution to cultivate enzyme-producing bacteria; Characterized in that the culture medium solution also contains corn flour and bean cake powder or lactose as secondary carbon sources and enzyme production inducers. The invention advances the enzyme production, improves the enzyme production speed, shortens the fermentation time and further reduces the production costs of amylase, protease and glucoamylase.
185 method for producing organic-inorganic compound fertilizer by using molasses fermentation waste liquid and filter mud
[Abstract] The invention relates to a method for producing organic-inorganic compound fertilizer by using molasses fermentation waste liquid and filter mud, which solves the problem of large-scale continuous recovery and treatment of molasses fermentation waste liquid. In the method, biological humic acid prepared from bagasse accounts for 65%-68% of filter mud, 10%- 12% of bagasse powder, 1.5%-2.5% of wheat bran and 16%-25% of regulator. After pile opening and drying, the fermented material with water content of 10% ~ 12% was obtained, and it was crushed into mud-filtering organic fertilizer. The molasses fermentation waste liquid with water content of 90% was concentrated into super concentrated solution with water content of 26% ~ 28% by three-effect forced circulation concentration system and two-effect scraping and steaming super concentration system. Uniformly mixing 30%-33% of ultra concentrated solution and 9%-10% of the above-mentioned biological humic acid according to weight percentage, then adding 32%-34% of chemical fertilizer and 25%-27% of filter mud organic fertilizer, stirring, dry extruding and granulating to obtain the organic-inorganic compound fertilizer.
208 fermentation and culture method, plant fermented extract, plant fermented extract composition, and method for preparing lipopolysaccharide and lipopolysaccharide
【 Abstract 】 In order to provide a cheap method for cultivating organisms with edible experience containing immune enhancers without using animal-derived ingredients, Acetobacter, Gluconobacter, Xanthomonas, Zymomonas or Enterobacter are cultivated in the culture solution with wheat or tofu waste as the main component, which is an edible Gram-negative bacteria with immune enhancement function. Therefore, Acetobacter can be obtained cheaply and safely, as well as low molecular weight lipopolysaccharide as immune enhancer. In addition, impurities derived from animal components are not mixed.
Method for produce active yeast high-protein feed by ferment alcohol waste liquid (residue) with 0 15 sucrose honey
Method for producing high-efficiency compound biological fertilizer by fermenting 258 sucrose filter mud
A culture medium for producing truffle polysaccharide by submerged fermentation.
237 Method for extracting chitosan from waste mycelium of Aspergillus terrestris produced by fermentation to produce itaconic acid
Erythritol fermentation process
26 1 method for produce xylanase by solid mechanical fermentation
238 plant saccharification and fermentation starter (bacterial protein feed) and new technology of alcohol fermentation production
Preparation of betaine from beet molasses fermentation waste liquid
Process for producing biogas by two-stage anaerobic fermentation of 086 citric acid waste sugar water
222 A method for producing butanol by fermentation of pentose and hexose.
Bacillus subtilis 065 and method for producing D- ribose by fermentation with the strain.
Method for producing ganoderma lucidum polysaccharide by fermentation in 2 15 circulating packed bed reactor
Method for preparing xylanase by submerged fermentation of 08 1 liquid
Method for comprehensively utilize 166 corn to produce starch sugar and residue-free fermented fuel alcohol
On-line sugar concentration detection system for 265 fermentation process
165 method for producing gulonic acid (VC precursor) from glucose by one-step fermentation
2 18 A strain for producing L- lactic acid and a method for producing L- lactic acid by synchronously saccharifying and fermenting Jerusalem artichoke with the strain.
188 method for continuous fermentation of glucose and xylose by coupling immobilized yeast cells with pervaporation membrane
030 fructose sodium diphosphate fermentation production method
194 Method for preparing L- ribose by biotransformation of ribitol fermentation broth
Method for preparing fermentation broth for biological hydrogen production by using sugar, protein and organic acid.
Method for preparing crystalline inorganic potassium salt and feed additive from molasses fermentation waste liquid
Iron pretreatment of 03 1 molasses wastewater biogas fermentation
Method for extract polysaccharide from Grifola frondosa 058 fermentation broth
Method for preparing xylitol by 040 biological mixed fermentation
Method for producing fuel ethanol by solid-state synchronous saccharification and fermentation of 093 sweet potato
A large number of fungal exopolysaccharides can be obtained by fermentation separation method of 170.
00 1 production method for producing ribonucleic acid by using bacteria in monosodium glutamate fermentation waste liquid
Method for manufacturing solid protein feed from 036 sugar-containing fermentation waste liquid
Aspergillus Niger 042 produces magnesium (manganese) gluconate from glucose.
Method for simultaneously producing hypocrellin and bamboo yellow polysaccharide by liquid fermentation
A method for producing avermectin by feeding sugar with OUR based on metabolic parameters.
164 method for extract fuel alcohol by direct enzymatic liquefaction acid saccharification and fermentation of sorghum
Method for co-producing arachidonic acid and chitosan by 252 biological fermentation
073 Fermentation method and fermentation system for preparing fuel alcohol with molasses as raw material
2 14 production of feed compound enzyme preparation by fermentation of waste liquid
262 A method for producing citric acid by fermenting sucrose hydrolysate.
098 pediococcus pentosaceus strain, starter made from the strain and its application in meat food.
Fermentation of 132 pentose
266 Chinese herbal medicine preparation fermentation box for treating diabetes
Method for using 22 1 molasses alcohol fermentation liquor as sugarcane liquid fertilizer
267 a Membrane bioreactor for 2- keto -D- gluconic acid fermentation.
Production of glucoamylase by Aspergillus Niger 20 1 pure fed-batch fermentation and its separation and concentration technology
193 fermentation method for improving polyporus umbellatus mycelium yield and polysaccharide content by polyporus umbellatus associated bacteria
Method for producing fermentable sugar by 2 13 enzymolysis of kenaf bast fiber
Method for preparing kefir polysaccharide by 150 biological fermentation
177 special strain for treating low-acid fermented meat products and preparation method of its starter.
Simultaneous fractional extraction of jujube flavonoids and jujube polysaccharides by 206 fermentation method
Application of 060 liquid sugar fermentation products in food
Fermentation preparation method of 169 ganoderma lucidum polysaccharide
Construction method and application of 247 xylitol fermentation recombinant yeast strain
0 19 ethanol production from sucrose using Zymomonas mobilis.
Strain 187 for producing bio-organic fertilizer by sucrose filter mud fermentation.
198 preparation method of xylitol and arabinose by biological fermentation
230 pure fermented low-alcohol and low-sugar fresh fruit juice yellow wine and its production method
Production of amino acids by direct saccharification of cassava and fermentation of sugar solution
Sterilization method of 236 sorbose fermentation medium by filter membrane
254. Method for manufacturing fermented beverage by using fermentation stock solution with low maltotriose content.
077 Medium Formula for Industrial Production of Rhamnolipid Fermentation Broth
Method for producing yeast extracellular trehalose by 006 two-step fermentation
163 method for producing starch sugar by sweet potato enzymatic acid and extracting fuel alcohol by residue-free rapid fermentation
Lactobacillus fermentum 248 and method for preparing D- tagatose by using same
004 Aspergillus Niger producing acidic β -mannanase and its fermentation and production method of mannooligosaccharides
167 method for directly produce starch sugar from sorghum and rapidly fermenting fuel alcohol without residue
022 new yeast strains for promoting sugar fermentation rate, methods for obtaining these strains and their applications.
06 1 method for producing ganoderma lucidum polysaccharide and ganoderma lucidum acid by intermediate fed-batch fermentation
Fermentation technology of 19 1 rhamnolipid biosurfactant
Process for preparing ribitol by 189 fermentation
Engineering strain 140 with mutant fructan sucrase gene of Zymomonas mobilis, and its construction and application
Method for separate and refining arabitol in 243 fermentation broth
Process for producing alcohol by efficient fermentation with waste molasses as raw material
079 low-sugar and low-fat semi-fermented health-care biscuit made of Coix seed and its processing method
124 large-scale submerged fermentation process for producing water extract and polysaccharide from Inonotus obliquus
Production method of lactic acid fermented by 037 high concentration hydrolyzed sugar
108 method for extracting ganoderma lucidum polysaccharide by solid fermentation
Aspergillus Niger 033 produces zinc gluconate from glucose.
1 19 method for producing astaxanthin by intermediate fed-batch fermentation with molasses or starch sugar as raw materials.
18 1 macromolecular polyphenols, therapeutic agents for mitochondrial diseases, preventive and therapeutic agents for diabetes, drinks and foods extracted from fermented tea.
Method for preparing rhamnolipid by biological fermentation
180 composition containing fermentable polysaccharide
High concentration fermentation technology of 25 1 2- keto -D- gluconic acid mixed salt
148 method for preparing nitrogen-containing syrup for fermentation by multi-enzyme method of rice and rice flour
Method for producing 2- keto -D- gluconic acid by 240 gluconate fermentation
02 1 improving the filterability of fermentation broth containing polysaccharides as a treatment method for auxiliary oil recovery.
Method for improving conversion rate and yield of sugar and acid in L- glutamic acid fermentation process
043 multifunctional saccharified fermented seeds (agent)
196 method for extracting polysaccharide from fermented cordyceps mycelium
178 method for preparing nitrogen-containing syrup for fermentation by multi-enzyme method of wheat B starch
Integrated fermentation and distillation process and device for producing ethanol from 232 sugar raw materials
122 submerged fermentation of Schizophyllum commune to produce polysaccharide and protein's anticancer method.
Method for producing alcohol by fermenting yeast resistant to high concentration of sugar and alcohol.
Method for preparing xylitol by 0 10 yeast fermentation
176 protective agent for improving the stability of α -galactosidase produced by Aspergillus fumigatus ZU-G 1 and its application
174 fermented tea, fermented tea extract, composition for inhibiting the increase of blood sugar level, and drinks and foods.
1 14 method for extracting d-ribose crystals from fermentation broth
Continuous fermentation technology of 209 2- keto -D- gluconic acid
127 method for producing d-ribose by fed-batch fermentation
055 A technological method for producing sugar by malt rice fermentation.
104 a method of separating and extracting d-ribose from fermentation broth by membrane separation technology.
2 16 method for producing fermentable sugar by sulfonation and separation of cellulose components and hydrolysis of lotus root synthase
Fermentation production method of 062 alkaline seaweed polysaccharide lyase and organism producing the enzyme
12 1 Organic fertilizer mainly composed of waste liquid and waste residue from sugar factory and decomposed starter and its production method.
A new technology for extracting sterols from waste residue after 145 sucrose honey fermentation.
Submerged fermentation technology of extracellular polysaccharide produced by medicinal fungus Rhizopus
Technology of simultaneous production of ganoderma lucidum polysaccharide and ganoderma lucidum acid by 050 liquid fermentation
Saccharification and Fermentation Technology of Eucommia ulmoides Oliv. 035
195 A low-alcohol fermented beverage wine for treating diabetes.
Purification method of 183 erythritol fermentation broth
Method for separating D- ribose from fermentation broth
Fermentation technology of 002 Agaricus blazei Murill and production method of its polysaccharide peptide
Fermentation production method of 224 liquid xylanase
Yeast strain 153 with improved fructose fermentation ability
Solid-state fermentation method and system for producing ethanol based on sugar raw materials
0 17 a high-sugar fermentation control process of biological glutamic acid with starch as raw material
123 a method to improve the conversion rate and yield of inosine during fermentation.
Eukaryotic cell metabolic engineering of xylose fermentation by Lian 179
Fermentation method of polyporus umbellatus strain 155 and production method of polyporus umbellatus polysaccharide
A new method for preparing 2- keto -L- gulonic acid from glucose by electric 039 fermentation
Metabolic engineering yeast Hua 226 for ethanol production by xylose fermentation.
Fermentation method of 0246 biological polysaccharide flocculant
2 147 Fermentation Technology for Producing Soybean Oligosaccharides with High Activity by Lactic Acid Bacteria
Method for treating erythromycin fermentation broth with 8 103 modified chitosan
|227 A method for producing D- ribose by biological fermentation
6 192 concentration method of sugar solution in cellulose ethanol fermentation
60 18 uses Zymomonas mobilis to convert sucrose into ethanol and other products.
Method for preparing selenium-enriched Flammulina velutipes crude polysaccharide powder by submerged fermentation of 907 1 liquid
2 154 Production of Rhamnolipid Crude Extract by Fermentation of Restaurant Waste Oil and Its Use
3 199 method for producing d-ribose by Bacillus subtilis fermentation
Method for producing D- ribose by fermentation of 3242 Bacillus pumilus transketolase mutant strain
1083 method for producing extracellular polysaccharide by liquid fermentation of tremella aurantialba and its application
Method for producing 1, 3- propanediol by 6 160 immobilized double bacteria in series glucose fermentation.
Osmotic controlled fermentation method for preparing acarbose by 038
Semi-continuous fermentation technology of 1203 2- keto -D- gluconic acid
8 100 Cellulose Oligosaccharide Fermentation Ferments Bacteria to Transform Organisms
9152 alpha-galactosidase solid fermentation production method
8259 fermentation medium and method for producing β -fructofuranosidase by Arthrobacter sp.
2 173 Glutamic Acid Fermentation Production Method Supplementing Mixed Carbon Sources of Molasses and Glucose
1 146 A method for producing β -mannanase by fermentation with Nostoc-like bacteria and its application.
9 1 10 saccharified seasoning koji and its application in fermented sauce products
Method for manufacturing organic compound fertilizer from 0028 molasses fermentation waste liquid
2 1 16 simultaneous saccharification and fermentation method and composition
Ganoderma lucidum 3005 with high polysaccharide yield and its fermentation production process
Method for obtaining high-purity D- ribose from glucose fermentation broth
234 saccharopolyspora rubra and method for producing erythromycin by fermentation thereof
244 A polysaccharide from Cs-4 fermented mycelium, and its preparation method and application.
Method for producing isopropyl alcohol by fermenting 0 16 glucose mother liquor
Extraction of Lycium barbarum polysaccharide by 134 fermentation
Method for preparing chitosan oligosaccharide and chitosan oligosaccharide by 158 coupling fermentation separation
099 pentose fermentation bacteria transform organisms
2 10 method for extracting ganoderma lucidum polysaccharide from fermentation broth
Method for producing fuel ethanol by simultaneous saccharification and fermentation of 085 Radix Puerariae
097 Staphylococcus xylosus I2 strain, compound starter made from the strain and its application in meat food
Fermentation method for producing pullulan by 088
Fermentation technology of Grifola frondosa 003 and production method of polysaccharide peptide thereof
Preparation method of corn starch syrup for 260 beer fermentation
Method for producing succinic acid by fermenting 184 molasses raw material
133 production method and products of polyporus umbellatus fermented bacterial powder and polyporus umbellatus polysaccharide
159 method for producing fuel alcohol by direct pulping of rice, enzyme liquefaction, acid saccharification and main fermentation.
14 1 Frozen dough containing pentosanase and lipase and its production method
2 1 1 compound yeast suitable for high concentration alcohol fermentation of sugar raw materials.
Penicillium Yang Qi strain producing α -galactosidase and its application in preparing α -galactosidase for feed by solid-state fermentation
229 Method of water plugging in oil wells by using Sclerotinia sclerotiorum polysaccharide fermentation broth
095 improved yeast fermented molasses treatment device
125 large-scale submerged fermentation process for producing Phellinus igniarius water extract and polysaccharide
068 distiller's grains mixed liquor fermentation to produce saccharifying enzyme and method for producing alcohol by fermenting kitchen waste with saccharifying enzyme.
172 method for producing lactic acid by saccharifying kitchen waste with Bacillus subtilis
092 Fructose-based Fermentation Raw Material and Its Application
Fermentation process of a new α -glucosidase inhibitor
New strain for producing D- ribose by fermentation of 034 and method for preparing D- ribose by using the strain
Regulation method of 1 15D- ribose fermentation process
249 A method for producing hyaluronic acid by fed-batch fermentation of sugar according to the acid yield.
A new technology of producing sugar by fermentation of 047 corn starch puree
2 12 Hericium erinaceus mycelium oligosaccharide extracted from Hericium erinaceus mycelium fermentation product and its application
Alcohol fermentation method of 029 sulfite pulping waste liquid rich in pentose
149 fermentation method for producing oligosaccharide-neotriose
Method for producing cellulase, xylanase, glucanase and pectinase by Trichoderma reesei 2 17 liquid fermentation
Industrial preparation method of 078 rhamnolipid biological fermentation broth
0 14 Streptococcus thermophilus prepared fermentation products rich in galactooligosaccharides and β -galactosidase.
0 13 Aspergillus Niger ferments glucose to produce gluconolactone
13 1 Method for producing frozen dough by freeze-resistant fermentation of trehalose-treated yeast
202 synthetic medium for liquid culture of Phellinus igniarius and method for producing Phellinus igniarius polysaccharide by fermentation.
09 1 Fermentation method for promoting biosynthesis of ganoderic acid and ganoderma lucidum polysaccharide
On-line sugar content detector for 094 beer fermentor
137 a method of separating residual sugar from organic acid fermentation broth and corresponding organic acid product mother liquor to purify organic acid.
144 method for improving biogas yield by xylanase
Method for preparing 2- keto -D- gluconic acid by fermenting 24 1 corn flour
Method for extracting ganoderma lucidum spore polysaccharide by puffing combined with fermentation
1 18 solid-state fermentation saccharified livestock and poultry feed and preparation method thereof
1 13 Bacillus coagulans strain SIM-7DSM 14043 is used to produce L(+)- lactic acid from fermentable sugars and their mixtures.
197 a method of adding sodium gluconate to improve acid production by fermentation.
Method for preparing fermentable reducing sugar from biomass
Method for separating and purifying erythritol from fermentation broth
Method for producing bacterial cellulose by utilizing 052 glucose oxidizing bacillus fermentation
120 large-scale submerged fermentation technology for producing Tricholoma matsutake and its polysaccharide
Raw material treatment method before saccharification when fresh potato raw materials are fermented to produce alcohol and alcohol products.
107 method for producing lactic acid or its salt by simultaneous saccharification and fermentation of starch
Treatment of raw materials before saccharification in the production of alcohol and alcohol products by fermentation
130 fermentation method for producing pullulan
Purification method of large-scale fermentation broth of 128 gene recombinant α -galactosidase
Zymomonas pentosaceus 109 strain and its application
15 1 gluconate fermentation broth used as cement and concrete admixture
182 method for fermenting sugar containing oligosaccharides
143 Method for producing L- amino acids by fermenting xylose with bacteria with enhanced gene expression
032 Food ingredients prepared by Saccharomyces boulardii fermentation and foods containing these ingredients.
1 17 composite accelerator for solid-state fermentation of saccharified feed for livestock and poultry
Biological fermentation method of 020 sugar-containing medium
Method for preparing selenium-enriched Agrocybe aegerita crude polysaccharide powder by submerged fermentation of 250 liquid
126 method of making fermented beverage from fermentation stock solution with low maltotriose content.
Method for producing starch sugar and rapidly fermenting fuel alcohol by directly dry grinding 168 rice
066 is a recombinant host suitable for simultaneous saccharification and fermentation.
Improvement of ethanol yield in mixed sugar fermentation with xylose in the presence of 233 sugar alcohol
106 method for extracting Coriolus versicolor glycopeptide from crude Coriolus versicolor glycopeptide obtained by submerged fermentation
23 1 method for synthesizing amino sugar modified bacterial cellulose from fermented coconut water
Method for controlling concentration of glucose and vitamins to improve acid production by fermentation
A liquid fermentation process of Agrocybe aegerita mycelium with high yield of polysaccharides and triterpenoids.
Fermentation production method of 082 solid acid xylanase
Technology of ethanol production by sugar juice fermentation of 069 sweet sorghum stalk
Fermentation preparation method of 139 trehalose
138 fermented soybean milk containing maltose
Fermentation technology for producing γ -linolenic acid with corn starch syrup as main raw material
16 1 Method for producing alcohol by fermentation of glucose and xylose *
157 lactose-free fermented soybean milk has effects of increasing bone density and inhibiting angiotensin converting enzyme, and its preparation method.
162 corn enzyme liquefaction, acid saccharification, two main fermentations, and intermediate extraction of alcohol to produce fuel alcohol with high yield and high speed.
263 A method for producing citric acid by sucrose fermentation.
Method for preparing nitrogen-containing starch syrup for fermentation by multi-enzyme method of 205 dehulled and degerminated corn flour
220. A method for preparing polysaccharide by fermenting kelp leftovers with Ganoderma strain.
Metabolic engineering transformation of eukaryotic cells to ferment arabinose
135 clean production technology for ethanol production by clear juice fermentation of sugar crops
186 method for promoting fermentation of waste molasses to produce lactic acid from food waste distiller's grains
Stable recombinant yeast 045 for xylose fermentation to ethanol
102 large-scale submerged fermentation process for producing Armillaria mellea mycelium powder and its polysaccharide
A dextrin fermentation medium for high mannanase production.
Method for producing N- acetyl -D- glucosamine by biological fermentation
053 production method for extracting caramel pigment raw materials by using waste alcohol mash and waste liquor of waste mash re-fermentation in sugar factory.
Biomass treatment method for obtaining fermentable sugar
156 method for producing xylanase by solid-state mechanical fermentation
129 Aspergillus niger with high activity of α-galactosidase and its solid-state fermentation process.
096 Lactobacillus pentosaceus strain, starter made from the strain and its application in meat food.
0 12 can effectively ferment glucose and xylose.
05 1 new strain and its application in the fermentation of 2- keto -L- gulonic acid
Method for checking and controlling metabolic pathway of aerobic glucose fermentation during lactic acid fermentation in mammals.
Method for producing succinic acid by synchronous saccharification and fermentation of 255 straw raw materials
Method for produce D- ribose by two-step fermentation
Fermentation method and equipment for improving theabrownin and polysaccharide components in Pu 'er tea.
175 lactose-free milkshake and/or almond syrup fermented products containing non-vegetable dried fruits.
Production technology of 044 corn coarse starch for sugar production and glutamic acid fermentation
026 saccharifying starter composition and application thereof
A technology for preparing ethanol by semi-solid fermentation of sugary crops.
1 1 1 Method for preparing xylitol by biological mixed fermentation
Production of polysaccharide solution by 027 biological fermentation
Extrusion method and device of 228 enzymolysis starch alcohol raw material and saccharification and fermentation method
Penicillium Yang Qi strain producing α -galactosidase and its application in preparing α -galactosidase for feed by solid-state fermentation
Method for producing high-protein feed by 136 sugar granule fermentation
Method for preparing culture medium rich in xylose and suitable for fermenting and producing xylitol
2 19 Lactobacillus helveticus strain that does not ferment lactose
0 1 1 The technology of brewing wine by enzymatic liquid saccharification and liquid fermentation with millet as raw material.
239. A method for producing extracellular polysaccharide from Ganoderma lucidum mycelium by two-phase supplementary sugar fermentation.
190 method for producing ethanol by simultaneous saccharification and fermentation of Jerusalem artichoke raw materials
Method for producing crude sugar raw material for 049 fermentation
142 frozen dough containing glucose oxidase and peroxidase and its production method
10 1 Method for recovering xylose from waste liquid of biomass hydrolysis and fermentation
Method for producing trehalose from fermentation waste