Rapid propagation of chrysanthemum in vitro
Li Wei Liu Liu Chengyun
(Wuhan Institute of Botany, China Academy of Sciences)
Quickly! embryo transplantation
INDICUMVAR. fragrance
Li Guangfan, Wei Zhengyuan, Liu Qihong, Liu Chengyun
(Wuha "I coarse st falsely accused T" TEOFBO Seven A "Y, Acade falsely accused a5 1 "falsely accused ea).
Keywords: Shennong Xiangju; In vitro rapid propagation
Keywords: sadness: spread from me
Experimental materials: chrysanthemum ([de:', a:t without e, a': "c ",(L.)DesMenl.var.aro"at "at.
Integrated circuits. Young leaves of willow blue and stem segments of test-tube seedlings.
The embedding conditions were based on MS, and 6-BA, NAA, 2.4-D and agar were added to the culture medium.
0.7%, sucrose 2%. Ph 8, culture temperature 23-30℃, day. The lighting lamp illuminates 8- 10 hour/day.
After surface sterilization, young leaves grew and differentiated, cut into small pieces of 0. 5×0. 5 mm2, and inoculated in MS 16-
BAZ.Omg/1(the same below) +NAAO. Take 5 as the medium. Light green compact callus grew after 10 days,
The induction rate is over 70%. Without transfer, callus can be directly differentiated into clustered buds on the original medium. In five days.
After that, rootless seedlings with high l.scm can be formed. The seedlings with callus were cut off and transferred to MS+6-BAI. O medium,
After 25 days, 8- 10 new seedlings can be regenerated from the base of each seedling with callus. To speed up replication,
The author also cut off the stems of test-tube plants and made them grow about 0. 5c. Small segments of internodes, cutted on the same fresh medium, each
Bottle materials can be divided into 3-4 bottles, and new buds can be seen in the axils of cuttings on the 5th day after grafting, and can grow after 20 days.
According to this method (that is, the method of cutting propagation in vitro), the seedling height is 2.ocm, which can not only save propagation greatly.
Breeding materials, and can obtain a large number of seedlings in a short time. In this experiment, after 5-6 generations of cutting culture, seedlings were obtained.
Can maintain the excellent economic characteristics of the original special fragrance of Shennong chrysanthemum.
Roots of seedlings grow very slowly in MS+6-BAI.0 medium, and only 1-3 fine roots can grow after 60 days.
Transplanting seedlings into MS+NAAO.3 medium can accelerate the growth of roots, and many strong roots can grow after 30 days.
There is no report on rapid propagation of chrysanthemum in vitro at home and abroad.
Textual research literature
Xiang Naibo plant physiology communications, 1986(4):40-4 1.
Liu, Chen Yinglan, Li. Wuhan Botanical Research,1986 (1):105-106.
Qiu Wenda plant physiology communications, 1983(6):39-40.
Very old ~ ~ ~
Discussion on 50 pages of construction organization design of landscaping project
South China Agriculture (Garden and Flower Edition)
No 12 of June 2007
Abstract: the construction organization design of landscaping project is that the construction unit completes any project.
An indispensable part of work. The tender of a construction enterprise usually consists of two parts: commercial tender and technical tender.
Composition, in the process of bid evaluation, the tenderee should not only consider whether the quotation in the commercial bid is reasonable and technical.
The content of construction organization design in the tender is also an important reference for measuring and selecting construction enterprises.
Foundation.
Keywords: garden; Planting project; Technical bid; Construction management plan
1 Importance of technical standards
For construction enterprises, technical tender refers to the manpower, material resources and financial resources that should be invested in bidding projects.
A reasonable plan is also a self-agreement to control the construction progress and ensure the construction quality, and it is also
Important written commitments to investors; For the construction party, it is used for supervision and inspection.
An important basis for checking project quality and mastering project progress. Therefore, high-quality technical tender not only
It embodies the ability of the construction unit in management and technology, and also ensures the smooth completion of the project construction.
An important prerequisite for this task.
2 Preparation before preparation of technical bid
2. 1 Learn the bidding documents and design drawings.
Carefully study the bidding documents and drawings, and carefully analyze the projects and works in the design drawings.
The relationship between them, the composition of materials and the collocation of seedling varieties.
2.2 Investigate the environment of the construction site
When doing technical bidding, we must fully understand and analyze the site situation where the project is located. Each project
There are different site conditions, and only after fully considering these objective factors can technical standards be formulated.
This book will have a clear goal.
2.3 Listen to the supplementary requirements of the tenderee for the project construction.
After fully understanding and clarifying the contents of bidding documents and design drawings, it is important to understand that investors have
What's the new intention? What other ideas are there subjectively? In my opinion, there is no violation of the provisions of the tender documents.
Under the premise that settlement does not affect the quality of engineering construction, it should be considered as much as possible.
Adopt the subjective intention of investors. The technical standards thus formulated will
More competitive and persuasive.
3 the composition of technical objectives
3. 1 project overview
Briefly explain the geographical location, total area and general situation of the project.
Block and so on. , have an overall description of the project.
3.2 Construction preparation plan
For bidders, it is necessary to make it clear to the bidding unit that the project is being implemented.
What preparations should the construction team make before entering the site? Main contents: such as
How to equip on-site project managers and other management personnel; Construction team's
Training and implementation; Procurement of main materials; Manager and
Implement the office and living rooms of construction personnel.
3.3 Construction technical scheme
This is the core content of the technical target, which should reflect the construction enterprise.
Technical level and management ability. In the technical bid, we must first work out
The construction process of the project should be scientific, reasonable and operable.
Generally, it is carried out according to the following procedures: site acceptance, site cleaning, soil entry and
Earthwork modeling, soil testing and improvement, fixed-point lofting, digging planting holes,
Plant large seedlings (including thinning and pruning before planting) and protect stumps and fields.
Level the site, plant seedlings, cover plants, clean up the site and raise the project.
Protection (including seedling transplantation) and handover. Then according to the construction process,
Make a detailed construction plan, and further elaborate that each working procedure should be
Master the technical points and precautions.
3.4 Construction progress plan
This content is usually expressed in the form of a table, where
Need to be clear about the construction time required for each project and which projects to implement.
Work needs to be done at the same time or cross. If there are no special circumstances, the table
The time listed is also the time required to complete the whole project. Do this
When observing, we should not only pay attention to the opinions of investors, but also consider objectivity.
Can not blindly meet the construction conditions and actual quantities.
At the request of investors, it violates the blind formulation of scientific and objective possibilities.
3.5 Human and material resources
The so-called allocation of manpower and material resources is the content decomposed according to the project.
Need to scientifically arrange labor and tools and equipment. Allocation of labor force
Not too much, lest people be overstaffed and increase labor costs.
Nor should it be too small to affect the progress of the construction period. Pay attention to when the labor force is equipped.
Italian skill matching, similarly, tools and equipment should not only be fully prepared, but also
And check its integrity and operating conditions.
3.6 Construction quality assurance measures
This paper mainly emphasizes how to ensure the project from both technical and management aspects.
Quality, usually including on-site technical management personnel, how?
Do a good job in design disclosure, ensure the construction according to the drawing, and establish quality inspection and acceptance.
Systems, etc.
3.7 Safe and civilized construction
Safety in production is related to people's life and material safety.
As an important part of bidding, the property of construction enterprises shall not be lost.
During the construction period, we must strictly abide by the management regulations of civilized construction.
According to the actual situation of the project, formulate corresponding safety management measures.
Materials shall be neatly stacked, and the construction area and living area shall be enclosed.
Environmental sanitation, pay attention to ensure the safety and reliability of on-site food procurement channels.
4 quality standards of construction organization design
There are three standards to measure the quality of construction organization design: (1)
There is obvious pertinence. (2) the integrity of the content. (3) It is operable.
Make love. There is no doubt that high-quality construction organization design should be clear.
Targeted, unique, only for the project. Landscape greening project
Construction organization design, should aim at the specific site conditions of the project, planting
Materials and planting time, so as to effectively guide and supervise.
Garden engineering construction, to ensure the quality and progress of the project. Similarly, high quality
Construction organization design should fully cover the whole process of the project to ensure the engineering quantity.
Integrity of the content. As for operability, in the construction organization design.
Try to quantify, avoid vague standards, and don't talk big and empty.
In short, whether the construction unit needs to design the construction organization or not.
As an integral part of the tender, the construction unit shall carry out detailed construction.
Organizational design. From the perspective of the construction unit, the construction organization design is
Guide the overall scheme of construction, which is used to control the quality and progress of the project; conform to the principle of simplicity
From the unit's point of view, construction organization design is a measure of project progress.
This standard is used to supervise the quality and progress of the project. Construction organization design is a job.
An important link in engineering construction is the importance of normal engineering construction.
Promise. Good construction organization design reflects the management level of the construction unit.
And technical strength; Effective construction organization design is the guarantee of the project.
Premises of quality and progress.