Shake the bacteria for 200 revolutions without resistance at 28℃ 12 hours. At this time, the OD can reach about 0.8~ 1. After 12 hours, measure the OD every 1 hour until the required OD= 1. Shake 16 hours if there are antibiotics, and then follow the above operation.

When shaking the bacteria, we must grasp the time and strength. As for how long it will take, it depends on the dosage. Generally, the more samples are taken, the longer it takes to shake the bacteria evenly.