1. Prepare 1 agarose gel (use 70ml for large gel and 50ml for small gel): weigh 0.7 g (0.5 g) agarose and place it in an Erlenmeyer flask, add 70 ml (50ml) 1×TAE, invert the mouth of the bottle into a small beaker. Heat in the microwave and boil for 3 times until the agarose is completely melted. Shake well to form 1.0 agarose gel solution.

2. Preparation of gel plate: clean the organic glass inner tank (gel making tank) in the electrophoresis tank, dry it, and put in the glue making glass plate. Use transparent tape to seal the glass plate and the inner tank. Seal the end edges to form a mold. Place the inner tank in a horizontal position and place the comb in a fixed position. Mix the agarose gel solution that has been cooled to about 65°C and carefully pour it onto the glass plate of the inner tank to make the glue The liquid slowly spreads until a uniform glue layer forms on the entire surface of the glass plate.

3. Let it stand at room temperature until the gel is completely solidified. Pull the comb vertically, remove the tape, and put the gel and inner tank into the electrophoresis tank. Add 1×TAE electrophoresis buffer until the gel plate is covered.

Extended information:

Storage temperature: normal temperature

There are many trade names, the common ones are, Sepharose (Sweden, Pharmacia), Bio-Gel-A (U.S.) Bio-Rad) etc. Agarose gel relies on secondary chains such as hydrogen bonds between sugar chains to maintain a network structure. The density of the network structure depends on the concentration of agarose. Generally, its structure is stable and can be used under many conditions (such as water, salt solutions in the pH range 4-9).

Agarose gel begins to melt above 40°C and cannot be sterilized by high pressure. It can be treated with chemical sterilization.

Agarose, abbreviated as AG, is an uncharged neutral component of agar, also translated as agarose or agarose. The chemical structure of agarose is composed of β-D-galactopyranose (1-4) connected to 3,6-anhydroα-L-galactopyranosyl units.

Agarose, that is, almost The main component of agar, which does not contain sulfate radicals, is polysaccharide. It is dissolved in hot water and cooled to form a gel. The resulting small particles are used in gel filtration. It is suitable for gel filtration of macromolecules that cannot be fractionated with sephadex. If a gel with a concentration of 5 or less is used, it can also fractionate cell particles, viruses, etc.

Taking advantage of its low adsorption characteristics, it is sometimes used instead of agar as a support for immunoelectrophoresis or in-gel sedimentation reactions.

Reference: Baidu Encyclopedia-Agarose Gel