The difference between calf serum and fetal calf serum;

1, different sources:?

(1) calf serum was taken from calves born at10-30 days, and blood was collected from the arteries of calves born at three months.

(2) Fetal calf serum is the fetal calf serum obtained by heart puncture and blood collection when pregnant cows are slaughtered.

2, the quality is different:?

(1) The quality of calf serum is not as good as that of fetal calf serum.

(2) Fetal calf serum is of the highest quality, because the fetal calf has not been exposed to the outside world, and the serum contains the least components harmful to cells such as antibodies and complements.

3. The purpose and usage are different:?

(1) calf serum and some cells only need calf serum.

(2) Some cells in fetal bovine serum need fetal bovine serum to grow.

4, the appearance is different:?

(1) calf serum is yellow and clear, slightly hemolytic, with foreign bodies and slightly viscous liquid.

(2) Fetal bovine serum is a character, with light yellow and clear appearance, no hemolysis, no foreign matter and slightly viscous liquid.

Extended data

1, the function of bovine serum:

Bovine serum is the most used natural culture medium in cell culture, which contains rich nutrients necessary for cell growth. It is often used for in vitro culture of animal cells and has extremely important functions.

(1) provides hormones for maintaining the exponential growth of cells, nutrients with little or no amount in the basic medium, and main low-molecular-weight nutrients.

(2) provide binding proteins, which can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the activity of the substances they bind.

(3) In some cases, protein can be combined with toxic metals and pyrogens to detoxify.

(4) It is the source of factors needed for cells to adhere to the wall and spread on the plastic culture medium.

(5) It acts as a pH buffer.

(6) Protease inhibitors are provided to inactivate the remaining trypsin during cell passage, thus protecting cells from damage.

2. Precautions for thawing serum: Avoid sediment.

(1) When thawing serum, please follow the suggested gradual thawing method (-2℃ to 4℃ to room temperature). If the temperature changes too much when thawing serum (such as -20℃ to 37℃), the experiment shows that it is very easy to produce precipitate.

(2) When thawing the serum, please shake it evenly at any time to make the temperature and composition uniform and reduce the occurrence of precipitation.

(3) Do not leave the serum at 37℃ for too long. If it is left at 37℃ for too long, the serum will become turbid, and many unstable components in the serum will be damaged, which will affect the quality of the serum.

(4) Thermal inactivation of serum is very easy to cause the increase of sediment, and this step is unnecessary unless necessary.

(5) If thermal inactivation of serum is necessary, please observe the principle of 56℃ for 30 minutes and shake it evenly at any time. Too high temperature, too long time or uneven shaking will cause the increase of sediment.

After the serum is thawed, flocculent precipitate is found, how to deal with it. To remove these flocculent deposits, the serum can be packed into sterile centrifuge tubes, slightly centrifuged at 400g, and the supernatant can then be added to the culture medium and filtered together. However, it is not recommended to remove these flocculent sediments by filtration because it may block the filtration membrane.

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