Cultivation method of horseshoe gold
Carry out soil preparation
Loosening, raking and fine grinding the ground to make the diameter of soil particles below 2 cm. Adjust the soil pH to 5.5~7.0. Use formalin: water = 1: 40 for soil disinfection, and the dosage is 10~ 15L per square meter of bed surface. The soil needs to be dried before disinfection.
Ditching transplantation
Open a small ditch with a depth of 2~3cm on the prepared bed with a spacing of 3~4cm. Separate the prepared calla lily plants, evenly place them in the ditch, keep the spacing between plants in the ditch 3~4cm, cover the ditch with 2cm soil, water it 1 time, cover it with sunshade net or grass curtain, and keep the soil moist every day. On the fifth day of planting, the plant grows vigorously, but the creeping branches have not fully contacted with the ground and grow new roots; On the day of 10, a large number of stolons will grow new roots and buds after fully contacting the ground.
Fine sand mulching cultivation
Before planting, water the prepared turf, manually tear the turf of horseshoe gold, spread it evenly on the turf, spread the prepared fine sand evenly on the horseshoe gold with a thickness of 6~ 10mm, keep the fine sand completely wet, and cover it with a sunshade net to prevent excessive evaporation of water. By the fifth day, a large number of new roots had grown on the stolon nodes of horseshoe gold; On day 10, a large number of new branches grew in the place where the new roots were born.
Breeding mode of horseshoe gold
Dichondra repens is often used for sowing and ramet cultivation.
Sowing propagation
The spring sowing date is April-May, and the autumn sowing date is 9-65438+1October. When the soil temperature reaches 265438 0℃, it is the best sowing date. The sowing amount is 10~ 15g/m2. Soak the seeds with 50mg/kgGA for 4 hours before sowing, and the effect is good. Drill or spread the treated seeds on the prepared bed, then rake the ground with a nail rake, so that the seeds are buried in the soil 1-2cm, pressed and covered with non-woven fabrics or grass curtains, so as to increase the germination temperature and maintain soil moisture, promote the orderly emergence of seedlings and improve the emergence rate.
Generally, spring sowing in March to April or autumn sowing in September ~10 ~15g/m2. Generally, it is planted after mixing with fine sand, covered with thin soil, and then covered with a layer of non-woven fabric, and watered carefully. It can germinate in 7~ 10 days. In fact, stolons are usually used for asexual reproduction. First, turn the ground deep and level, remove sundries, apply sufficient base fertilizer, then spread grass blocks, slightly cover with fine sand or fine soil, roll and water.
Split propagation
From April to May, the stolons with soil will be scooped up, divided into small stems and planted according to the hole spacing 15-20cm. Plant water to moisturize and pay attention to weeding.
Pharmacological effects of dichondra repens
1, protecting liver and reducing enzyme
Pharmacological experiments show that the extract of water chestnut root has certain therapeutic and protective effects on acute liver injury caused by carbon tetrachloride * * * CCl4 * *, and can obviously reduce the serum transaminase increased in mice with liver injury within 1 week and 3 weeks; It can alleviate the pathological changes of liver and increase the rate of white ball after liver injury. The experiment with fresh grass juice of Dictyophora dictyophora showed that it reduced the contents of alanine aminotransferase ***ALT*** in serum and malondialdehyde * * * MDA * * in serum and liver tissue in a dose-dependent manner, significantly increased the level of superoxide dismutase * * * SOD * *, and significantly reduced the degeneration and necrosis of liver cells, so it had obvious protective effect on carbon tetrachloride and acute liver injury caused by carbon tetrachloride. ALT is released through the dissolution of mitochondrial membrane lipids in damaged hepatocytes. The drug can reduce the permeability of cell membrane and inhibit the release of intracellular enzymes, thus improving liver injury and promoting the recovery of liver function. The content of malondialdehyde reflects the degree of tissue peroxidation. SOD is an antioxidant enzyme in the body, which can scavenge free radicals and prevent them from damaging the cell structure. The fresh juice of horseshoe root can reduce the content of malondialdehyde, increase the activity of superoxide dismutase and reduce the lipid peroxidation of liver, thus protecting the liver.
For D- galactosamine ***D-Glan***, thioacetamide * * TAA * *, isothiocyanate-1- naphthyl ester ***ANIT***, it can obviously reduce serum transaminase and triglyceride in the liver of mice with D-Glan liver injury, and alleviate the pathological changes of liver tissue; It can reduce serum alanine aminotransferase in mice with liver injury, reduce serum total bilirubin * * * TBIL * * and serum transaminase ***ALT and AST*** levels in mice with cholestatic jaundice, improve experimental jaundice liver injury, promote liver function recovery, make bilirubin excrete normally, and play the role of protecting liver and eliminating jaundice; It can also significantly reduce the content of triglyceride * * * TG * * in liver tissue. In the anti-HBV experiment, the maximum non-toxic concentration of the compounds ***2R, 3r * *-2,3-dihydroxy -2- methyl-γ-butyrolactone is 0.4 μmol/ml, and the inhibition rate of HBsAg is 22.4%, and the inhibition rate of HBeAg is 19.6%, which has certain inhibition effect on HBV. The effective components of dichondra repens and their specific cellular and molecular biological mechanisms need further study.
2. Analgesic effect
Mice were treated with writhing method, hot plate method and electroacupuncture method, and the pain threshold of mice was determined with different doses of horseshoe extract. The results show that the extract of water chestnut root has good analgesic effect.
3. Anti-inflammatory effect
Small dose of water chestnut extract can obviously inhibit the increase of capillary permeability caused by acetic acid; Inhibitory effect of carrageenan on toe inflammation and swelling in rats. Both water chestnut root extract and its petroleum ether extract can obviously inhibit acute inflammatory edema of mouse auricle caused by xylene, but the anti-inflammatory effect of water chestnut root extract petroleum ether extract has no obvious dose-dependent relationship, and the middle dose has always had good pharmacological activity on different inflammatory models.
4, antibacterial effect
Microbiological experiments showed that the extract of water chestnut root had strong antibacterial effect on Gram-positive pathogenic cocci such as Staphylococcus aureus and Streptococcus hemolyticus B, but weak inhibitory effect on Gram-negative bacilli such as Escherichia coli, Salmonella typhi, Proteus and Aeromonas. It has no effect on Shigella flexneri.
5, antipyretic and cholagogic effects
Large dose of water chestnut root extract * * 32.5 g/kg * * * can obviously reduce the fever body temperature of rats caused by peptone for a long time, and has a good antipyretic effect. The bile flow increased significantly within 120 minutes after a small dose of horseshoe root extract * * 8.2g/kg * * * was injected into the duodenum of rats, which indicated that the drug had a strong cholagogic effect.
6, the role of enhancing immunity
Small dose of horseshoe gold can obviously increase the weight of thymus and spleen, enhance the phagocytic function of mononuclear macrophages, and increase the level of serum hemolysin, which shows that the drug has the effect of promoting cellular immunity and humoral immunity.
7, anti-cancer effect
The high content of selenium in horseshoe gold was determined by HG-ICP-AES. Elemental selenium has high antioxidant activity, which is 50~500 times that of vitamin E. It can scavenge free radicals in the body, prevent organ aging and pathological changes, delay aging, enhance human immunity and resist the toxic effects of toxic heavy metals. Studies have proved that selenium can inhibit the growth of cancer cells and the synthesis of DNA, RNA and protein, inhibit the transcription of oncogenes, and interfere with the metabolic process of carcinogens in the human body, thus effectively fighting cancer. Pharmacological effects of dichondra repens