peroxidase, enzymology classification number is EC 1.11.1.7. Donor+ H2O2--→Oxidized donor+2 H2O。

Horseradish Peroxidase,HRP) is the most commonly used enzyme because of its high specific activity, stability, small molecular weight and easy preparation. HRP is widely distributed in the plant kingdom, with high content in horseradish. It is a glycoprotein composed of colorless enzyme protein and brown iron porphyrin, with a sugar content of 18%. HRP is composed of several isoenzymes, with a molecular weight of 4, and an isoelectric point of 3 ~ 9. The optimum PH for enzyme catalysis varies slightly with different hydrogen donors, but most of them are around PH5. The enzyme is soluble in water and ammonium sulfate solution with saturation below 58%. The maximum absorption spectra of HRP's auxiliary group and enzyme protein are 43nm and 275nm, respectively, and the purity of the enzyme is generally expressed by the ratio RZ (German Reinheit Zahl) of OD43nm /OD275nm. The RZ value of high-purity enzyme should be around 3. (up to 3.4). The smaller the RZ value, the more nonenzymatic proteins. 1） RZ> 3 activity > 25u/mg, mainly used in immunology, is a high-purity peroxidase. Special chromatographic purification technology was used to remove the isoenzyme B .

2) RZ> 2 activity >; 18u/mg, mainly used in clinical chemistry, and our customers have also applied this specification to immunological research. At this point, a standardized analysis method becomes particularly important.

3） RZ> 1 activity > 1u/mg, mainly used in blood glucose test paper and urine analysis test paper.

4） RZ> .6 activity >; 6u/mg, mainly used for urine analysis test paper.