The loading buffer contains glycerol or sucrose, which increases the density of the sample. Only by increasing the density can your sample be denser than TAE when you sample, so that the sample will settle in the sampling hole.

The role of goldview nucleic acid dye is to stain DNA, and it can be seen clearly after running the gel. They have different functions. Use all of them.

The safest thing now is gelred nucleic acid dye. Can replace EB.

The United States has done relevant cell experiments, and EB and others can easily enter cells, but gelred can't. Moreover, gelred is non-toxic, and the US Environmental Protection Agency has confirmed that it can be directly discharged into the sewer! . There are many fakes on the market, many of which are blended by EB. So check the biotium in official website, USA. Or check China, official website, Beijing, Bosheng and Philip Burkart.