One, the experimental environment
A professional plant tissue culture laboratory is one of the key factors for the success of plant tissue culture. The whole process of plant tissue culture must be carried out in a sterile environment, from the disinfection of the explants, pruning, inoculation to the later sterile seedling cultivation, all can not be separated from the sterile experimental environment, a professional and clean plant culture laboratory is indispensable to the success of plant tissue culture experiments, which can greatly reduce the contamination of the tissue culture operation and the sterile seedling cultivation process.
Two, experimental equipment and consumables
1. Ultra-clean bench: is a kind of air purification equipment to provide a high degree of local cleanliness of the working environment of the generality of the strong, is the core of the instrument in the culture of plant tissues, can provide the degree of cleanliness of up to one hundred levels. Without the aseptic environment provided by an ultra-clean bench, the success rate of plant tissue culture would be virtually nil. In a conventional laboratory environment, it is impossible to carry out plant tissue culture operations, and even if the experimental operations are completed, the final sterile seedlings will be contaminated by external microorganisms. The Chuanbran Ultra-clean bench has a UV protection device, which is a national patent protected product. This device can prevent students from being harmed by UV light when they forget to turn off the UV light in case of improper operation, which is a necessary "weapon" for the success of plant tissue culture.
2. High-pressure steam sterilizer: it is another core instrument for plant tissue culture experiments, which sterilizes the culture medium and inoculation instruments during the process of plant tissue culture, so as to achieve the purpose of sterility. In the 2012 Beijing Chuanbran Group Culture Competition, because some schools did not purchase autoclaves, but used ordinary household autoclaves to sterilize the culture medium (the sterilization temperature of household autoclaves could not reach the temperature of killing bacteria completely), the final experimental results showed that the probability of contamination reached more than 70%, which resulted in the failure to complete the Group Culture Competition; and most of the schools used autoclaves to sterilize the culture medium and combined with ultra-clean workbenches. While most of the schools that use autoclave sterilization and combined with the use of ultra-clean bench can effectively control the chance of contamination in less than 10%, some schools even reach zero pollution, in short, ultra-clean bench and autoclave is the decisive instrument for the success or failure of the plant tissue culture, and the choice of a professional histoculture necessary equipment is more conducive to the development and success of the experiment.
3. Micro-adjustable pipette and pipette tip: the experimental tools for measuring micro-liquid, used for measuring small amount and micro hormone mother liquor, adjusting the concentration of hormone needed in various culture media. Micro-adjustable pipette in other experiments in the application rate is also high, and some experiments require the instruments used to ensure sterility, that is, can be autoclaved and ultraviolet irradiation, traditional pipettes can not be accomplished, and by the Kawabulan micro-adjustable pipette in the design and development of the full consideration of this point.
4. Light culture frame: A place for sterile seedling culture, providing the light needed for plant growth and controlling the light intensity according to the actual situation. If the inoculated histoculture bottles are directly placed in the general environment, the induction will fail due to insufficient light intensity. Therefore, it is preferable to choose a light culture frame to provide more suitable growth conditions for the histocultured seedlings.
5. Light incubator: A place for sterile seedling cultivation, which can not only provide appropriate light intensity in the process of plant culture, but also control the temperature of sterile seedling cultivation. In addition, the light incubator can also be adjusted according to the growth habit of different plants, adjust the light cycle, suitable for extracurricular exploration activities, for example, short-day chrysanthemum varieties by adjusting the length of time of light can be controlled by the chrysanthemum flowering or raising insects, small animals, and so on. Light incubator is more powerful than the light culture frame, the application surface is not only limited to the scope of plant tissue culture, and the management is more convenient, even in the case of unattended summer and winter, as long as the parameters are set up beforehand, there are no worries.