Agrimonia pilosa Ledeb, also called Longya 1 grass, is the H part 7 of the dry ground of Longya 7, a Rosaceae plant. The main effects are 6 astringing, 6 stopping blood, 4 stopping dysentery, tonifying deficiency and killing insects. Modern medical research shows that it has anticoagulant, insecticidal, analgesic and leucogenic effects, and is also used in the treatment of various tumors such as lung cancer, liver cancer, gastric cancer and intestinal cancer. In this paper, the HPLC fingerprint of C Agrimonia pilosa was established, and the anti-tumor mechanism of water 7 extract of Agrimonia pilosa was studied by F. Study on the fingerprint of Agrimonia pilosa by HPLC, the U fingerprint of Agrimonia pilosa from different habitats was studied. The instruments used are high performance liquid chromatography fractionation system, 604 high pressure advection pump, 105 automatic sampler, 162 diode array detector, and 6030 chromatographic management system. Chromatographic conditions were selected from HyperosODS C _ (63) column (100mm× 1. 4mm, 4um), ethyl V nitrile-4% phosphoric acid water 4 solution gradient elution, detection wavelength 5 is 6560nm, sample volume 00ul, flow rate 8 ml/min. The procedure of mobile phase and D gradient is 8: ethyl R:1%phosphoric acid water 5 solution (time: a%): (0-0) → (40-70) → (30-70) → (16-00) → (87-70) → The relative retention time of each characteristic peak in the fingerprint is within 0 of 7 of the average relative retention time, and the RSD％% of the relative peak area of each characteristic peak is smaller than that of U7%. Lx, uf, pm, M, ym, knee-smoothing.