1, structural difference: brassinolide is a kind of sterol with four-ring structure, which contains hydroxyl group and two methyl groups; Brassinolide is a lactone with a five-membered ring structure.

2. Different functions: Brassinosterol has many biological activities, such as antioxidation, antibacterial and anti-inflammatory, and has a certain health care function; On the other hand, brassinolide has biological regulation function and participates in the process of plant stress resistance and growth and development.