(1) We should select the parts that should be sterile under normal conditions and collect samples with strict aseptic technique, such as blood, pleural effusion, deep pus, bile, etc. ?

(2) Specimens collected and submitted for inspection should use a special anaerobic specimen bottle, or the specimen should be directly sucked into a syringe to exhaust air, and the needle should be inserted into a sterile rubber stopper to avoid contact with air; ?

(3) The culture medium should be fresh and inoculated in time, preferably at the bedside; ?

(4) Select a qualified anoxic culture environment.