Blood agar plate (BAP) is the most commonly used enrichment (nutrient) medium, which can isolate many bacteria with high nutritional requirements, such as Streptococcus, Enterococcus, etc.; because it can be observed on BAP Different hemolytic types of bacteria can also be used for the culture and identification of bacteria with low nutritional requirements, such as Staphylococcus aureus, Pseudomonas aeruginosa, etc.

BAP and chocolate culture medium are made from the same raw materials, except that the latter is prepared at a higher temperature, which destroys red blood cells and releases nutrients in red blood cells, such as X, V factors, etc., so chocolate culture The base is more nutritious and can culture Neisseria gonorrhoeae, Neisseria meningitidis, Haemophilus influenzae, etc., but because the red blood cells have been destroyed, hemolysis cannot be observed.