Glycyrrhiza uralensis Fisch is a commonly used bulk medicinal material. With the development of the times and the deepening of its research, the application field of Glycyrrhiza uralensis Fisch is expanding, which leads to the increasing demand of Glycyrrhiza uralensis Fisch, far exceeding its output. At present, the production of cultivated licorice has been carried out in the natural distribution area of wild licorice. Therefore, there are both cultivated licorice and wild licorice in today's medicinal market, but whether there are differences in quality between them, whether the curative effects are the same, and whether the curative effects of cultivated licorice from different provenances, different producing areas and different growth years are the same are all problems that must be solved in clinical application of cultivated licorice. In this study, the contents of various effective components in Glycyrrhiza uralensis Fisch were determined and their HPLC fingerprint characteristics were analyzed. Based on the results of some pharmacodynamic experiments, the quality characteristics of different cultivated and wild Glycyrrhiza uralensis Fisch. produced in Hangjinqi, Chifeng and Baicheng of Jilin Province under the current cultivation techniques were analyzed and evaluated, and the following results were obtained: 1. The main fingerprint peaks of cultivated licorice and wild licorice were analyzed by HPLC-MS, and it was found that the retention time before 22min was mainly dihydroflavone glycoside, chalcone glycoside and flavone glycoside. Triterpenoid saponins predominate within 25-35 minutes, and coumarins predominate after 35 minutes. 2. The study on the influence of producing area on the quality of cultivated Glycyrrhiza uralensis Fisch found that (1) affected the content of four effective components in medicinal materials, and the statistical results showed that the influence of producing area on glycyrrhizic acid and polysaccharide was very significant; There was no obvious effect on total flavonoids and liquiritin. Hangjinqi in Inner Mongolia has the highest content of the four components, while Chifeng in Inner Mongolia and Baicheng in Jilin have little difference. (2) The producing area affects the relative proportion of the four active ingredients. (3) The relative proportion of flavonoid glycosides and coumarin also shows different laws due to different producing areas. The relative content of coumarin and other compounds in Baicheng, Jilin Province is relatively high. The proportion of coumarin and other components in Chifeng sample is very low. (4) The quality characteristics of cultivated Glycyrrhiza uralensis Fisch in three different producing areas were clarified. 3. The study on the effect of growth years on the quality of cultivated Glycyrrhiza uralensis Fisch found that (1) growth years had a significant effect on the content of total flavonoids and polysaccharides in medicinal materials. The contents of glycyrrhizic acid, liquiritin and total flavonoids in medicinal materials basically increased or remained unchanged with the extension of growth years in 2 ~ 4 years, which was basically positively correlated with the years. However, the content of polysaccharide is negatively correlated with the growth years, and decreases with the increase of growth years. In the 5-year-old samples of Hangjinqi, Inner Mongolia, it was found that the contents of various components decreased. (2) The growth years have no obvious influence on the relative proportions of glycyrrhizic acid, liquiritin, total flavonoids and polysaccharides in Glycyrrhiza uralensis Fisch, but have great influence on the content of flavonoid glycosides in total flavonoids. The relative proportion of their contents increases with the increase of growth years, that is, the proportion in the total components increases; The proportion of coumarin in total components has no obvious change; Isoliquiritigenin decreased with the growth time. 4. The study on the influence of provenance on the quality of cultivated Glycyrrhiza uralensis Fisch found that provenance had little influence on the content of three effective components in the sample, mainly affecting flavonoids. 5. The study of different wild Glycyrrhiza uralensis Fisch in Hangjinqi found that the contents and proportions of (1) four effective components in wild Glycyrrhiza uralensis Fisch with red and green stems on the ground were close, and the difference was not obvious. The similarity of HPLC fingerprint is also very high; (2) The content of glycyrrhizic acid and liquiritin in the root of Glycyrrhiza uralensis Fisch was significantly higher than that in the rhizome, but the content of total flavonoids and polysaccharides was not significantly different. (3) The contents of four effective components in different grades of medicinal materials have nothing to do with grades; (4) Compared with Glycyrrhiza uralensis Fisch with special structure (samples containing only taproot), the content of glycyrrhizic acid in Glycyrrhiza uralensis Fisch is significantly different, and the content of glycyrrhizic acid in Glycyrrhiza uralensis Fisch is higher than that in Glycyrrhiza uralensis Fisch with special structure. But there is no obvious difference in liquiritin, total flavonoids and polysaccharides. 6. Comparing the quality of cultivated Glycyrrhiza uralensis Fisch and wild Glycyrrhiza uralensis Fisch, it is found that (1) the contents of liquiritin, total flavonoids and polysaccharides in most cultivated Glycyrrhiza uralensis Fisch are similar to those in wild Glycyrrhiza uralensis Fisch, but the contents of glycyrrhizic acid are obviously different; (2) The content of each component of 3-5 year-old cultivated Glycyrrhiza uralensis Fisch in Hangjinqi, Inner Mongolia is similar to that of wild Glycyrrhiza uralensis Fisch, but the relative content of liquiritin is higher; (3) The proportion of four effective components in wild licorice is relatively stable. However, the proportion of four components in cultivated licorice is related to the place of origin. From the HPLC fingerprints of wild and cultivated Glycyrrhiza uralensis Fisch, it is found that the peak shape and retention time of most fingerprint peaks in the two fingerprints are basically the same, and the chemical components are also basically the same. After the retention time of cultivated Glycyrrhiza uralensis Fisch and wild Glycyrrhiza uralensis Fisch was 35min, there were obvious differences among the four chromatographic peaks, that is, one chromatographic peak of wild Glycyrrhiza uralensis Fisch was not obvious in the chromatogram of cultivated Glycyrrhiza uralensis Fisch, while three chromatographic peaks of cultivated Glycyrrhiza uralensis Fisch (including liquiritin, liquiritigenol and isoliquiritigenol) were not obvious in the wild Glycyrrhiza uralensis Fisch. Therefore, these two groups of peaks can be used to preliminarily distinguish wild products from cultivated products. 7. Pharmacodynamic study on protecting liver and reducing enzyme of wild and two cultivated Glycyrrhiza uralensis Fisch. With different quality characteristics, it was found that (1) for acute liver injury caused by carbon tetrachloride, although the contents of glycyrrhizic acid and liquiritin in the three tested herbs were quite different, there was no difference in the experimental results. And there is no difference in liver protection and enzyme reduction among the three dosage groups of each medicinal material, and there is no clear dose-effect relationship. (2) Different qualities of Glycyrrhiza uralensis Fisch have different antibacterial effects. From the overall bacteriostatic effect of several strains in the experiment, the three-year-old licorice cultivated in Hangjinqi is greater than or equal to the wild green stalk > three-year-old licorice cultivated in Chifeng > four-year-old licorice cultivated in Baicheng. The antibacterial effect is related to the content of glycyrrhizic acid and liquiritin. 8. The analysis of the relationship between natural conditions and glycyrrhizic acid content in three experimental sites shows that higher temperature and stronger sunshine are beneficial to the formation and accumulation of glycyrrhizic acid; The moisture condition is contrary to the above temperature, and precipitation and air humidity are negatively correlated with glycyrrhizic acid content. The content of glycyrrhizic acid is also related to the soil conditions in geographical location. The analysis of 18 element in medicinal materials and their growing soil also found that the content in medicinal materials is directly proportional to the content in the corresponding soil. And the higher the content, the stronger the regularity; Due to different habitats and environments, the contents of various elements in four kinds of licorice are quite different. In short, wild and cultivated licorice have their own characteristics; From the results of this study, the influence of producing area on the quality of Glycyrrhiza uralensis Fisch is greater than the growth years and provenance. The quality of cultivated Glycyrrhiza uralensis Fisch meets the requirements of current national standards and is suitable for production in the producing area. Even those licorice plants that have only grown for 2 years can be used scientifically and reasonably as long as we master their quality laws. For example, cultivated licorice. And licorice. The content of coumarin and liquiritol in Glycyrrhiza uralensis Fisch. produced in Baicheng is high, which can meet special needs. The characteristics and innovations of this paper are as follows: (1) Quality research and evaluation methods of Glycyrrhiza uralensis Fisch. (2) The influence of producing area and growing time on the quality of Glycyrrhiza uralensis Fisch was further revealed. (3) Find out the main similarities and differences between the fingerprints of wild and cultivated licorice; (4) The quality characteristics of cultivated Glycyrrhiza uralensis Fisch from three different producing areas were clarified; (5) The efficacy of the same batch of Glycyrrhiza uralensis Fisch with different quality characteristics was preliminarily evaluated.