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What is the function of agarose gel electrophoresis markers?
The function of agarose gel electrophoresis labeling is molecular labeling.

The advantages of DNA molecular markers are: most molecular markers are dominant, which is very convenient for the selection of recessive traits; Genome variation is extremely rich, and the number of molecular markers is almost infinite; At different stages of biological development, DNA from different tissues can be used for marker analysis; Molecular markers reveal the variation of DNA;

It is neutral, does not affect the expression of target traits, and has no linkage with unhealthy symptoms; The detection method is simple and rapid. With the development of molecular biology technology, there are dozens of DNA molecular markers, which are widely used in genetic breeding, genome mapping, gene location, species relationship identification, gene bank construction, gene cloning and so on.

Extended data

Marker, a region on a chromosome that can be recognized (such as restriction enzyme cut points, gene positions, etc.). ). The inheritance of markers can be detected. Markers can be parts of chromosomes that have expression function (such as genes), or parts that do not encode protein function but whose genetic characteristics can be detected.

Protein markers can be divided into:

The unstained marks are wide molecular weight protein standard, high molecular weight protein standard and low molecular weight protein standard;

Pre-dyeing marks include monochrome pre-dyeing and multi-color pre-dyeing.

In the west? In the process of imprinting, the molecular weight mark is like a screw. Although it is a small detail, it is such a small detail that plays an important role in the experimental results.

Members of the protein imprinting reference family are mainly used to indicate the molecular weight of the protein band. Only when the standard quantity is accurate can the experimental results be convincing. In addition, protein standard can also indicate the success of transfer or the electrophoresis degree of protein on the gel, so choosing the correct protein marker is also one of the necessary conditions for the success of western blot experiment.

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