1, bacterial culture medium
Formula 1 beef paste agar medium
Beef paste 0.3g, peptone1.0g, sodium chloride 0.5g, agar1.5g,
Water 1000 ml
Add water 100 ml into the beaker, add beef paste, peptone and sodium chloride, mark the beaker with crayon, and heat it on the fire. After the components in the beaker are dissolved, add agar and stir constantly to avoid sticking to the bottom. When agar is completely dissolved, make up the water loss, adjust the pH value to 7.2 ~ 7.6 with 10% hydrochloric acid or 10% sodium hydroxide, subpackage in each test tube, add cotton plugs, and sterilize with high pressure steam for 30 minutes.
Formula II Potato Culture Medium
Take 250g of fresh beef heart (excluding fat and blood vessels), finely chop it into minced meat with a knife, and then add 500ml of distilled water and 5g of peptone. Mark the beaker, boil it and simmer for 2 hours. Filtering, drying the filtered minced meat, and adjusting the pH value of the filtrate to about 7.5. Add 10 ml broth and a small amount of minced dried beef heart into each test tube, and sterilize for later use.
Formulated rhizobium culture medium
Glucose10g dipotassium hydrogen phosphate 0.5g.
3 g of calcium carbonate and 0.2 g of magnesium sulfate.
0.4g yeast powder and 20g agar.
Water 1000 ml 1% crystal violet solution 1 ml.
Firstly, the agar is boiled and dissolved in water, then other components are added separately, stirred and dissolved, packaged and sterilized for later use.
2. Actinomycete culture medium
Formula 1 Starch agar medium (Gauss medium)
Soluble starch 2g potassium nitrate 0. 1 g
0.05g dipotassium hydrogen phosphate and 0.05g sodium chloride.
Magnesium sulfate 0.05g ferrous sulfate 0.00 1 g.
Agar 2g water 1000 ml
First, put the starch in a beaker, mix it into paste with 5 ml of water, then pour in 95 ml of water, stir well and add other drugs to dissolve it. Mark the outside of the beaker, add agar when it is heated to boiling, and keep stirring. After the agar is completely dissolved, make up the water loss. Adjust the pH value to 7.2 ~ 7.4, subpackage and sterilize for later use.
Formula II Flour Agar Medium
60g flour and 20g agar.
Water 1000 ml
Mix flour into paste with water, add water to 500 ml, and cook on slow fire for 30 minutes. Take another 500ml of water, put it into agar, heat and boil until it is dissolved, then mix the two solutions evenly, replenish water, adjust the pH value to 7.4, subpackage and sterilize for later use.
3. Fungal culture medium
Formulation of Sabouraud's culture medium
20g of peptone10g agar.
Maltose 40g water 1000 ml
Firstly, peptone and agar are added with water, then heated and continuously stirred. After the agar is dissolved, 40 grams of maltose (or glucose) is added, stirred and dissolved, and then packaged and sterilized for later use.
This culture strain is commonly used to cultivate many kinds of fungi.
Formula II Potato Sugar Agar Medium
Wash and peel the potatoes, cut 200 grams into small pieces, add water 1000 ml, boil for half an hour, and make up the water. Add10g agar to the filtrate, boil and dissolve, then add 20g sugar (sucrose for mold culture and glucose for yeast culture), make up water, package and sterilize for later use.
Adjust the pH value of this culture medium to 7.2 ~ 7.4, and the sugar in the formula, such as glucose, can also be used to cultivate actinomycetes and Bacillus.