abstinence time should be 2 ~ 7 days, preferably within 3 ~ 5 days. The number of abstinence days should be counted from the last ejaculation (whether sexual intercourse, nocturnal emission or masturbation). The length of abstinence time will mainly affect the amount of semen and sperm density, but has little effect on sperm morphology and motility.
if abstinence time is too short, semen volume is often less, and sperm density is also low. However, if the abstinence time is too long, the sperm death rate may increase due to the prolonged storage time in epididymis, which can not reflect the normal situation. Abstinence for 3 ~ 5 days basically conforms to the sexual law of most people, and the semen test results are close to my physiological situation.