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Application of high performance liquid chromatography

1 Used as a means of analysis

Divided into two forms of use alone and in conjunction with other analytical equipment.

The application range of stand-alone use has been very wide, is a routine instrumental means of analysis, and there are many related national standards, and more than 600 of them were being implemented in 2015.

For example:

GB/T 30388-2013 Determination of total capsaicinoid content of chili peppers and their oleoresins High-performance liquid chromatography

GB/T 25224.2-2010 Determination of soybean steroidal diene in animal and plant fats and oils Vegetable oils and oils Part 2: High-performance liquid chromatography

GB/T 17528-2009 Pepperine Determination of piperine content High performance liquid chromatography

The use of supporting mainly with mass spectrometry and other equipment, can effectively determine the mixture of samples or unknown components of the sample.

For example:

GB/T 29664-2013 Determination of Vitamin B3 (Niacin, Niacinamide) in Cosmetics High Performance Liquid Chromatography and High Performance Liquid Chromatography Tandem Mass Spectrometry

GB/T 23217-2008 Determination of Tetrodotoxin in Aquatic Products Liquid Chromatography - Fluorescence Detection

2 Used as a means of trace separation. For details, see the term "preparative chromatography"

It is called high-performance liquid chromatography. It is used to separate trace amounts of substances by collecting them separately after they have been separated.

This is very common in the preparation of high-end chemicals, such as chiral drug intermediates.