2. Pharmacopoeia Standard of Niuhuang Qingxin Pill (Bureau Prescription) 2. 1 Name Niuhuang Qingxin Pill (Bureau Prescription)
Niuhuang Qingxin Pill
2.2 prescription bezoar 25.7g, angelica sinensis 45g, Ligusticum chuanxiong 39g, licorice150g, yam 2 10/0g, scutellaria baicalensis 45g, fried bitter almond 37.5g, soybean yellow roll 57g, jujube 90g, fried atractylodes rhizome 75g, poria cocos 48g, platycodon grandiflorum 39g and Saposhnikovia divaricata 45g. 75g of Massa Medicata Fermentata (parched), 54g of Cinnamomum cassia, 44g of Ophiopogon japonicus, 22.5g of Ampelopsis, 7.5g of Pollen Typhae (parched), 6.4g of artificial musk, 0/6 of borneol1g of buffalo horn concentrated powder, 28.4g of antelope horn and 69 of cinnabar.
2.3 The preparation of the above twenty-nine flavors, except bezoar, artificial musk, borneol and buffalo horn concentrated powder, cinnabar and realgar are respectively crushed with water; Grinding antelope horn file into fine powder; The other 22 yam are crushed into fine pow; Grinding concentrated powder of Calculus Bovis, artificial musk, Borneolum Syntheticum and Cornu Bubali, mixing with the above powder, sieving, and mixing. Every110g of powder, add 90-100g of refined honey to make honeyed pills, or add water (add 4% refined honey) to make pills to make watered pills.
2.4 Characteristics This product is a reddish-brown honey pill or water pill; It smells fragrant and tastes slightly sweet.
2.5 Identification (1) Take this product and observe it under the microscope: the irregular branched mass is colorless and soluble in chloral hydrate test solution; The mycelium is colorless or light brown, with a diameter of 4 ~ 6 microns (Poria cocos). The diameter of calcium oxalate clusters is 18 ~ 32μ m, which exists in parenchyma cells and is often arranged in rows, or a cell contains several clusters (Paeonia lactiflora). The needle-like crystals of calcium oxalate are fine, with the length of 10 ~ 32 microns, and irregularly filled in parenchyma cells (fried atractylodes). The needle-like crystal bundles of calcium oxalate are 24 ~ 50 microns long and exist in round or oval mucus cells (Ophiopogon japonicus). The parenchyma cells around the fiber bundle contain calcium oxalate crystals, forming crystalline fibers (licorice). The phloem fiber is light yellow, spindle-shaped, thick-walled, with pores and grooves (Scutellaria baicalensis Georgi). The diameter of the milk-separating tube is 65438 0.4 ~ 25 microns, and it contains pale yellow granules (Platycodon grandiflorum). Stone cells are orange-yellow, shell-shaped, thick-walled, and have obvious pits (fried bitter almonds) on the wider side. Epidermal cells of pericarp are yellowish brown to reddish brown with polygonal surface, and the cross section of cuticle is about 10μm (jujube). The palisade cells in the seed coat are light yellow and 45 ~ 80μ m long (soybean yellow roll). Pollen grains are yellow, round or oval, with a diameter of about 30μm, and there are tiny warty protrusions (pollen typhae) on the outer wall. Irregular fine particles are dark brown-red, shiny, and the edges are dark black (vermilion). Irregular fragments are golden yellow or orange yellow with luster (realgar). Irregular fragments are grayish white or light grayish yellow, slightly shiny, with grayish brown pigment particles on the surface and irregular longitudinal cracks (buffalo horn concentrated powder).
(2) Take this product 10 pill, cut into pieces, take about 1.2g, add the same amount of diatomite, grind, add 50ml of methanol, heat and reflux for 3h, evaporate the extract, add 5ml of ethanol to the residue for ultrasonic dissolution, centrifuge, and take the supernatant as the test solution. In addition, a proper amount of cholic acid and hyodeoxycholic acid were taken as reference substances, and ethanol was added to make a solution containing 0.5mg per kloc-0/ml as reference substance solution. According to the test of thin-layer chromatography (Appendix ⅵ b of Pharmacopoeia Part I, 20 10), 5ul of the test solution 10ul and the above two reference solutions were accurately absorbed, respectively, and placed on the same silica gel G thin-layer plate, and the upper solution of cyclohexane-ethyl acetate-methanol-acetic acid (20: 25: 3: 2) was used as the developing agent for two times. They were detected under sunlight and ultraviolet (365nm) respectively. In the chromatogram of the test sample, spots of the same color and fluorescent spots appear at the positions corresponding to the chromatogram of the cholic acid reference substance. [ 1]
(3) Take 6g of honey pills of this product, cut into pieces, add 3g of diatomaceous earth, and grind them evenly, or take 4g of water pills, grind them evenly, add 30ml of ether, ultrasonic wave for 65438 00 minutes, filter, stand by and watch the filtrate, evaporate the ether from the filter residue, add 30ml of methanol, ultrasonic wave for 30 minutes, filter, evaporate the filtrate, and add 65438±05ml of water to the residue. Another baicalin reference substance was added with methanol to prepare a solution containing 65438±0mg per 65438±0ml as the reference substance solution. According to the test of thin-layer chromatography (appendix ⅵ b of Pharmacopoeia I, 20 10), absorb 4 ~ 6 μ l of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, and use ethyl acetate-butanone-formic acid-water (5: 3: 1: 1) as the developing agent, unfold and take them out. In the chromatogram of the test sample, spots with the same color appear in the position corresponding to the chromatogram of the control sample.
(4) Take this product 15g honey pill, chop it, add 7g diatomite, and grind it evenly, or take 8g water pill, grind it evenly, add 50ml chloroform, perform ultrasonic treatment for 30min, filter, discard chloroform solution, volatilize the solvent in the residue, add 50ml water to saturate n-butanol, perform ultrasonic treatment for 40min, filter, wash the filtrate with 3 times of ammonia test solution, and separate the n-butanol layer. In addition, take ginsenoside Rb 1 reference substance, ginsenoside Re reference substance and ginsenoside Rg 1 reference substance, and add methanol to prepare a mixed solution containing 1mg per 1ml as reference substance solution. According to the test of thin-layer chromatography (Appendix ⅵ b of Pharmacopoeia I, 20 10), absorb 4 ~ 6 μ l of the above two solutions, spot them on the same silica gel G thin-layer plate, and put the lower solution in chloroform-methanol-water (65: 35: 10) below/kloc-0℃/kloc. In the chromatogram of the test sample, spots with the same color appear in the position corresponding to the chromatogram of the control sample.
(5) Take the ether extract under (3) (identification), evaporate the ether, and dissolve the residue with 0.5 ml of ethyl acetate as the test solution. Take 0.5g of Angelica sinensis, add 20ml of diethyl ether, ultrasonicate for 65438 00 minutes, filter, evaporate the filtrate, and dissolve the residue with 0.5ml of ethyl acetate as a control medicinal solution. According to the test of thin-layer chromatography (Appendix ⅵ b of Pharmacopoeia I, 20 10), absorb 6 ~ 10μ l of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, develop with n-hexane-ethyl acetate (9: 1) as the developing agent, take them out, dry them in the air, and place them in an ultraviolet lamp (365nm). In the chromatogram of the test sample, fluorescent spots with the same color appear at the positions corresponding to the chromatogram of the control medicinal materials.
2.6 Check 2.6. 1 hyodeoxycholic acid, and test according to the method under [Identification ](2). In the chromatogram of the test sample, the spots with the same color and fluorescent spots shall not be displayed in the position corresponding to the chromatogram of the hyodeoxycholic acid reference substance. [ 1]
2.6.2 Others shall comply with the relevant provisions under Pills (Appendix 1A of China Pharmacopoeia I, 20 10).
2.7 Indications: clearing away heart-fire, resolving phlegm, relieving convulsion and expelling wind. It is used for dizziness, excessive phlegm, unconsciousness, slurred speech, convulsion, epilepsy and other symptoms caused by wind and phlegm blocking collaterals.
2.8 Oral administration and dosage. Honey pill 65438+ 0 pills at a time, water pill 65438+ 0.6g at a time, once a day 1 time.
2.9 Pay attention to pregnant women with caution.
2. 10 water pill? Weight of every 20 grains1.6g;
Big honey pill? Each pill weighs 3 grams.
2. 1 1 storage seal.
Version 2. 12