The antithrombotic effect of Radix Paeoniae Rubra 15-20g (crude drug) /kg was given to rats by gavage, which obviously prolonged the thrombosis time, shortened the length and reduced the weight. Prothrombin time and partial thromboplastin time of kaolin were prolonged, and euglobulin lysis time was shortened, which indicated that it had a significant inhibitory effect on blood coagulation. Radix Paeoniae Rubra extract 125-250mg (crude drug) /ml not only significantly prolonged prothrombin time and partial thromboplastin time, but also significantly inhibited thrombin from agglutinating bovine fibrinogen, and it was completely non-coagulated at 500 ~1000 mg /ml. In addition, it can activate plasminogen and dissolve coagulated fibrin, so Radix Paeoniae Rubra plays an antithrombotic role by inhibiting thrombin and activating plasminogen. Paeonia lactiflora (d- Catechin) 200 mg/rat, once a day 1 time for 46 days, significantly prolonged the platelet aggregation time, platelet thrombosis time and thrombosis time in rats fed with high-fat diet, and the thrombus length and wet weight were significantly lower than those in control group. Radix Paeoniae Rubra extract can also improve the hemorheology of patients with coronary heart disease with high blood viscosity, which can reduce the whole blood viscosity, prolong the erythrocyte electrophoresis time and reduce the platelet aggregation at medium and low shear rates. Radix Paeoniae Rubra extract tablets can significantly reduce the whole blood viscosity, plasma viscosity and hematocrit of patients with pulmonary heart disease.
Anti-platelet aggregation effect of Radix Paeoniae Rubra extract significantly inhibited platelet aggregation induced by adrenaline, adenosine diphosphate (ADP), welding head snake venom (TMVA) and arachidonic acid (AA) in vitro, and decreased platelet adhesion and platelet factor III activity, and increased the content of cAMP in platelets. Radix Paeoniae Rubra and its derivatives 80 1, 802 in 5? l0-4～5? L0-3ml/L has obvious inhibitory effect on ADP and collagen-induced platelet aggregation in rabbits, of which 802 has the strongest effect, and it still has depolymerization effect on ADP-induced platelet aggregation. The anti-platelet aggregation effects of the drugs are consistent with the effects of reducing the oxygen consumption of rat liver mitochondria, respiratory control rate and phosphorus/oxygen (P/O) ratio, indicating that the anti-platelet effects of the three drugs are related to interfering with the energy metabolism of platelets. Radix Paeoniae Rubra essence inhibited the biosynthesis of thromboxane A2(TXA2)-like substances when rabbit platelet-rich plasma (PRP) was incubated with AA, which significantly reduced the contraction amplitude of its products on rabbit aortic strips, indicating that the inhibition of platelet aggregation by Radix Paeoniae Rubra essence was related to the promotion of TXA2. Intravenous drip of Radix Paeoniae Rubra essence to patients with coronary heart disease and angina pectoris can significantly reduce the percentage and aggregation number of enlarged platelets, indicating that the increased platelet surface activity and aggregation can be significantly reduced. The above experiments show that the mechanism of Radix Paeoniae Rubra inhibiting platelet aggregation may be related to interfering with the energy metabolism of platelets, inhibiting the biosynthesis of TXA2 and increasing the content of cAMP in platelets.
Anti-arteriosclerosis effect of Radix Paeoniae Rubra extract tablet 5g (crude drug) /kg, taken/kloc-0 once a day for/kloc-0-1 5 weeks, to make the plasma total cholesterol (Tch), triacylglycerol (TG), low density lipoprotein cholesterol (LDL-Ch) and very low density in rabbits with hyperlipidemia. High density lipoprotein cholesterol (HDL-Ch) and HDL-2-CH were significantly higher than those in the control group. Tch/HDL-ch, tch/HDL-2-ch, LDL-ch/HDL-ch and LDL-ch/HDL-2-ch ratio decreased significantly, and the ratio of thromboxane A2/ prostacyclin (TXA2/PGI2) caused by hyperlipidemia tended to be normal. In addition, it can reduce plasma lipid peroxide (LPO), arterial wall lipid, calcium and phospholipid, and aortic plaque area. The anti-atherosclerosis mechanism of Paeonia Rubra may be related to improving the ratio of lipoprotein components, reducing plasma lipid peroxide, reducing calcium deposition on arterial wall, inhibiting platelet aggregation, regulating TXA2/PGI2 balance and activating adenylate cyclase to increase cAMP concentration in arterial wall.
Effect of Radix Paeoniae Rubra on Cardiovascular System: Radix Paeoniae Rubra (Radix Paeoniae Rubra) decoction slightly inhibited the isolated toad heart and rabbit in situ heart, and slowed down the heart rate and stroke volume l：0.5 The large dose (1:0.5, 0. 15ml) obviously inhibited and had conduction block. Vascular perfusion slightly dilates the internal organs, hind limbs and isolated rabbit ears of toad. Perfusion of isolated rat hearts with 0.2% Radix Paeoniae Rubra injection increased coronary flow by 28.4%. Intravenous injection can not only increase coronary flow, but also reduce peripheral resistance and blood pressure. These effects are not affected by reserpine pretreatment, and may be direct. Injection of Radix Paeoniae Rubra into the abdominal cavity of mice increased the uptake of 86Rb in myocardium, which indicated that the nutritional blood flow of myocardium was increased, and this effect could be inhibited by propranolol, which indicated that it was related to? -receptor-related. To some extent, it can alleviate and improve the early cardiac function decline after scald in rats, and Paeonia Rubra injection 10g (crude drug) /kg can be given orally. Intramuscular injection of Paeonia Rubra Injection 1g/kg has therapeutic and preventive effects on experimental pulmonary hypertension in rabbits, which can dilate pulmonary vessels, improve pulmonary blood flow, reduce pulmonary artery pressure, increase cardiac output and improve cardiac function.
Radix Paeoniae Rubra injection can also dilate pulmonary vessels, reduce pulmonary artery pressure and pulmonary vascular resistance, increase cardiac output, improve right heart function and hemorheology in patients with pulmonary heart disease. Caoshaoyao extract tablets also have similar effects on patients with pulmonary heart disease. 40g (crude drug) /kg and 80g (crude drug) /kg of Paeonia Rubra injection were injected intraperitoneally, which obviously prolonged the survival time of mice under atmospheric hypoxia. 8g/kg intravenous injection has obvious protective effect on acute myocardial ischemia induced by pituitrin in rats. 40g/kg gavage can also prolong the survival time of mice under decompression and hypoxia.
Anti-tumor effect of Radix Paeoniae Rubra (Paeonia Rubra D)1~ 2g/kg was injected intraperitoneally, and the inhibition rate of S 180 solid tumor in mice was 3 1%-49%. The water extract of Radix Paeoniae Rubra (Radix Paeoniae Rubra A) or 70% ethanol extract (Radix Paeoniae Rubra C) alone has no obvious effect on S 180 solid tumor, but it has obvious inhibitory effect when combined with subthreshold dose of cyclophosphamide. Radix Paeoniae Rubra A and C have no obvious effect on leukemia in 6 15 mice, but when combined with low dose methotrexate, the survival time of 6 15 mice can be significantly prolonged. The content of cAMP in tumor tissue is obviously lower than that in normal tissue. Paeonia lactiflora A, C and D can increase the level of cAMP in S 180 solid tumor, S 180 ascites cancer or Lewis lung cancer respectively, which may be related to anti-cancer or anti-cancer synergistic effect. The plasma fibrin content of S 180 mice with solid tumor is 2-3 times higher than normal. The intraperitoneal injection of Paeonia lactiflora A2g/kg can significantly reduce it, which may also be related to the anti-cancer synergistic effect. Radix Paeoniae Rubra A or C alone can promote the spontaneous metastasis of Lewis lung cancer, and low dose cyclophosphamide has no obvious effect, but the combination with Radix Paeoniae Rubra A can significantly reduce this lung metastasis.
Protective Effects of Radix Paeoniae Rubra (Radix Paeoniae Rubra) Injection (3.3mg/ml, 1.67mg/ml and 0.7mg/ml) can obviously promote DNA synthesis of cultured hepatocytes in vitro, and has a good effect on hepatocyte regeneration and liver function recovery. Intravenous injection of 3.75g/kg of Radix Paeoniae Rubra has obvious protective effect on liver injury induced by D- galactosamine in rats, which increases the survival rate of animals, liver atrophy and alanine aminotransferase are significantly lower than those in the control group, and FN (the main opsonin of monocyte-macrophage system) is higher than that in the control group. The mechanism of liver protection may be to increase the level of plasma fibronectin (PFN) in rats, thus enhancing the phagocytic function of reticuloendothelial system and the activity of opsonin, so as to protect hepatocytes, prevent liver immune damage and promote hepatocyte regeneration. Paeonia lactiflora 80 1, 24mg/kg, once a day 1 time for 2 weeks, can obviously induce the activity of carboxyesterase (CEase) in the liver and heart of rats, which can promote the hydrolysis process in vivo and promote the excretion of poisons in vivo. D- Catechins 100mg/kg or 50mg/kg intraperitoneal injection has protective effect on acute injury of liver mitochondria in mice induced by ethanol, which can reduce the lipid fluidity of liver mitochondria membrane in model animals, increase the content of reduced glutathione (GSH) in liver mitochondria, reduce Ca+uptake and lipid peroxide production, and alleviate the acute injury of liver mitochondria membrane structure by antagonizing the toxic effect of ethanol.
Propyl gallate, a derivative of gallic acid, a component of Radix Paeoniae Rubra, has the ability of scavenging oxygen free radicals, can obviously inhibit mitochondrial swelling and lipid peroxidation induced by ferrous sulfate and vitamin C, and can protect the normal structure and function of mitochondria. Radix Paeoniae Rubra has no obvious effect on normal isolated guinea pig ileum, but it can relieve intestinal spasm caused by acetylcholine. Subcutaneous injection of 3g/kg of n-butanol extract from Paeonia Rubra can promote the phagocytosis of reticuloendothelial system and increase the weight of liver, but has no obvious effect on hemolysin production and delayed hypersensitivity. Radix Paeoniae Rubra extract, right? -hydroxyl-? -methylglutaric acid coenzyme A and calcium channel blocker receptor have significant inhibitory effects. Radix Paeoniae Rubra has a strong inhibitory effect on Shigella dysenteriae, Salmonella typhi and Streptococcus hemolyticus in vitro. The decoction of Paeonia lactiflora can temporarily increase the blood sugar of rabbits, and the water-alcohol extract of Paeonia lactiflora has obvious sedative and analgesic effects, and can also improve the acidity of gastric juice, appetite and digestive function.
Clinical application of Radix Paeoniae Rubra