Electrophoresis is generally divided into two categories: free interface electrophoresis and zone electrophoresis. Free interface electrophoresis does not require supports, such as isoelectric focusing electrophoresis, isokinetic electrophoresis, density gradient electrophoresis and microelectrophoresis, etc. This kind of electrophoresis is rarely used at present. Zone electrophoresis, on the other hand, requires various types of substances as supports, and commonly used supports include filter paper, acetate fiber film, non-gel support, gel support and silica gel-G thin layer, etc. The most commonly used in the field of molecular biology is agarose gel electrophoresis. The so-called electrophoresis refers to the movement of charged particles in the electric field, different substances due to the different charges and molecular weights, so the movement speed in the electric field is different, according to this feature, the application of electrophoresis can be qualitative or quantitative analysis of different substances, or will be a certain mixture of component analysis or a single component extraction and preparation, which is of great significance in the clinical examination or experimental research. Electrophoresis instrument is designed and manufactured based on the above principle. The following is a brief introduction to its use and precautions.
● How to use
1. First, connect the two electrodes of the electrophoresis tank with the DC output of the electrophoresis instrument with a wire, and pay attention to the polarity not to be reversed.
2. Adjust the power switch of electrophoresis apparatus to the off position, turn the voltage knob to the minimum, and select the mode of voltage and current stabilization and the range of voltage and current according to the working needs.
3. Turn on the power, slowly rotate the voltage knob until the desired voltage is reached, set the termination time of electrophoresis, and then electrophoresis will start.
4. After finishing the work, turn the knobs and switches to zero position or off state, and dial out the electrophoresis plug.
Precautions
1. After the electrophoresis instrument is energized and enters the working state, it is forbidden for the human body to touch the electrodes, electrophoresis and other possible charged parts, and it is not allowed to pick up and put things in the electrophoresis tank, if necessary, it should be disconnected first, so as to avoid electric shock. At the same time, it is required that the instrument must have a good grounding end to prevent leakage of electricity.
2. After the instrument is energized, don't increase or remove the output wire plug temporarily to prevent short-circuit phenomenon, although there is a fuse attached to the instrument, the short-circuit phenomenon may still lead to instrument damage.
3. Due to the different resistance values of different media supports, the electrophoresis through the flow of electricity is also different, and its swimming speed and swimming to the end of the time required is also different, so the electrophoresis of different media supports should not be carried out at the same time on the same electrophoresis instrument.
4. When the total current does not exceed the rated current of the instrument (the maximum current range), it can be used in multiple tanks, but be careful not to overload, otherwise it will easily affect the life of the instrument.
5. When checking the electrophoresis input of the instrument under some special circumstances, it is allowed to start the machine under no-load in the state of stabilized voltage, but the load must be connected before starting the machine in the state of stabilized current, otherwise, the needle of voltmeter will jump greatly, which is easy to cause unnecessary man-made damage to the machine.
6. During the use of abnormal phenomena found, such as large noise, discharge or abnormal odor, must immediately cut off the power supply, maintenance, in order to avoid accidents.
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