Mushroom seeds are crushed, dried at low temperature, accurately weighed, degreased by ether reflux, ether extract recycling, mushroom seeds are added to 20 times the amount of water, the temperature is 50 ℃, the reaction time is 80 min to extract the mushroom polysaccharide crude, activated charcoal decolorization [ 5 ], centrifugation to remove the precipitate, the supernatant is added 95% ethanol, so that the final volume fraction of ethanol 75%, centrifugation to take the precipitate that is obtained mushroom polysaccharide. The crude product was obtained by centrifuging the precipitate.

The crude mushroom polysaccharide was dissolved in water and passed through a DEAE2 52 column (cm × 60 cm, column height 52 cm), which was first equilibrated with phosphate buffer pH 518, and the sample was dissolved in this buffer and loaded onto the column.

The elution procedure was as follows: first, 400 mL of distilled water was eluted, and then 80 mL of phosphate buffer was used in a gradient elution.

The sample was eluted with a gradient of phosphate buffer.