The main function of fetal bovine serum is to cultivate cells. . .
I hope I can help you to introduce the composition and function of serum below.
First, fetal bovine serum
All the quality indicators meet the requirements of the third appendix of the 2005 edition of the Chinese Pharmacopoeia.
1, properties, light yellow and clear appearance, no hemolysis, no foreign matter and slightly viscous liquid.
2. protein content is 3.5% ~ 5.0% (w/v).
3. Hemoglobin content ≤ 0.02%( w/v)
4. Sterility test is negative
5, mycoplasma test negative
6. Bacterial endotoxin ≤5(EU/ml)
7. Bovine diarrhea virus is negative
8, Escherichia coli phage negative
9. Growth curve (inoculation concentration) supporting cell proliferation (Sp2/0-Ag 14) The maximum proliferation concentration is ≥2.5× 106 cells /ml.
Cell doubling time ≤ 15h
The cloning rate is ≥85%
Main components of serum
Serum is a very complex mixture formed by removing fibrin from plasma. Although most of its components are known, some of them are still unknown, and the composition and content of serum often vary with the gender, age, physiological conditions and nutritional conditions of blood donors. Serum contains various plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc., which are in physiological balance for promoting cell growth or inhibiting growth activity.
Fetal calf serum should be taken from the fetal calf delivered by caesarean section; Newborn bovine serum is taken from newborn cattle within 24 hours of birth; Calf serum was taken from calves born10-30 days.
Obviously, the fetal calf serum is of the highest quality, because the fetal calf has not been exposed to the outside world, and the serum contains the least harmful components to cells such as antibodies and complements.
function
Bovine serum is the most used natural culture medium in cell culture, which contains rich nutrients necessary for cell growth. It is often used for in vitro culture of animal cells and has extremely important functions. 1. provides hormones for maintaining the exponential growth of cells, nutrients that are absent or small in the basic medium, and main low-molecular-weight nutrients. 2. Provide binding proteins that can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the activity of the substances they bind. 3. In some cases, protein can combine with toxic metals and pyrogens to detoxify. 4. It is the source of factors needed for cells to adhere to the wall and spread on plastic culture medium. 5. It acts as a pH buffer. 6. Protease inhibitors are provided to inactivate the remaining trypsin during cell passage and protect cells from damage.
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