Cordyceps sinensis is mainly produced in alpine areas and snowy grassland in Qinghai, Tibet, Sichuan, Yunnan, Gansu, Guizhou and other provinces in Chinese mainland.
Artificial cultivation method of Cordyceps sinensis
(1) Cordyceps sinensis
1. Improved mother medium formula: peptone 1%, glucose 10%, yeast extract 0. 1%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0. 1%, agar 2% and water/kloc-. Inoculated into a triangular bottle or wine bottle with this culture medium, and planted at room temperature of 15-20 degrees for one week, it can be seen that hyphae grow around tissue fragments, and the growing hyphae are not vigorous; After 2 months, colonies were formed with tissue fragments as the center; After 3-4 months, the vertical diameter of the colony can reach 1-2 cm, the colony is protruding, the surface is uneven, and it is gray to yellowish brown; The colony is compact and hard, and the matrix mycelium is brown-black with black pigment. After continuous culture on solid medium for 6 months, the offspring can grow, and their shapes are very similar to those grown in the field.
2. The field cultivation of Cordyceps sinensis can be divided into the preparation of seed (spore) solution. Site selection Three parts of liquid culture.
(1), preparation of spore suspension: the formula is: potato 200g, sugar 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1. 5 grams, vitamin 10 tablets, zengchanling 0. 5 grams. The collected Cordyceps spores are put into this liquid culture medium to make spore suspension.
(2) Site selection: Cordyceps hepialus larvae usually overwinter in loose soil. The requirements for soil are high sediment concentration, less soil and good ventilation. You can refer to this standard when choosing the location of Cordyceps sinensis. If it is cultivated indoors, it is necessary to set up multi-layer wooden frames, and each layer is covered with 7 cm thick sand with bamboo boards or sawdust. The humidity of sand is kept at about 40%, and the soil is covered with leaves or sundries. Generally, it is most suitable to be used as a room facing south as a cordyceps culture room.
3. Liquid culture: Cordyceps sinensis can only be cultured when the host insect develops to the second larval stage. The culture method is to dilute the spore suspension to the concentration of 5- 10%, and then spread it evenly on each insect. It should be done on cloudy days or at sunset, or around 8 pm. In the harvest season (June-July), you can see that the soil is exposed at the top of the sub-seat.
4. Bottle cultivation: Bottle cultivation is a good method, which is easy to manage and transfer over a long distance. Jars or pottery pots are commonly used, and the culture material is "vegetable garden soil plus 15% soil, or yellow soil plus 10% sediment, ph 6-7". Mix the soil well and bottle it. Be careful not to fill it so full that the larvae can't get in. Fill it and put it in the shade. Cover the bottle with leaves. Gather two larvae together and smear spore suspension. In order to improve the success rate, it can be divided into two times, every 8 hours and 24 hours, according to the proportion required by the size of the dissolver. Check it every day for the next 7 days to prevent accidents. Entities can be collected in summer, washed and dried with water, and sealed for sale.