The artificial cultivation method of puffballs of the present invention belongs to the technical field of cultivation of edible fungi and solves the problems of artificial domestication and cultivation of wild puffballs. It includes tissue separation, seed preparation, ingredients, inoculation, and development. Bacteria and mushroom production management, the cultivation method is carried out as follows: first ingredients, that is, according to the following formula and weight ratio, weigh 5-15% cotton kernel powder, 2-10% wheat bran, 42-50% sawdust, and 10-10% fruit leaves. 35%, cow dung 5-15%, potassium dihydrogen phosphate 2-5%, gypsum 1-2%, lime 1%, mix the above raw materials thoroughly, adjust the moisture content to 60-65%, and stack the culture material for fermentation Immediately after 25-30 days, bag and plant or fill the mushroom shed for planting in the sowing ditch. After inoculation, the bacteria will grow in the mushroom shed. The temperature of the mushroom shed should be controlled at 16-26°C, and the lighting should be low light or dark. The first tide After harvesting the mushrooms, clean the bed surface and spray water once. After 15-20 days, harvest the second tide of mushrooms, manage the third and fourth tides of mushrooms, and so on. This method takes about one day from puffball seeding and acclimation to harvesting. In about a year, 2-3kg of puffballs can be harvested per square meter.