1, culture technology.

Chicken fir fungus through the liquid deep culture mycelium, as a raw material to provide the development of nutritious food and beverage, its protein content is higher than other mushrooms, especially lysine and leucine content is very high, so the chicken fir fungus liquid deep culture mycelium production attracted attention.

Medium formula applicable liquid medium formula:

Formulation 1: protein knee 2%, sucrose 2%, magnesium sulfate 1.5%, potassium dihydrogen phosphate 0.3%, vitamin B11 mg/100 ml, pH adjusted to 6.

Formulation 2: yeast paste 0.1%, sucrose 3%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 1%, sodium nitrate 0.3%, potassium chloride 0.05%, pH adjusted to 6.

Formulation 3: yeast paste 0. 1%, sucrose 3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.5%, sodium nitrate 0.3%, potassium chloride 0.05% p H6 or so (Hong Zhen, China Medical University, 1992).

2. Nutrition.

The main source of nutrition for the growth and development of wild chanterelles is obtained from ant nests and fungus beds and the surrounding environment. Ant nest is mainly by the worker ants of semi-digestive swarming well heat becomes the fungus bed, or fungus bed, the fungus bed is rich in **** nutrients, including lignin, cellulose, fat, a variety of amino acids and ash elements. Together with the minerals and water around the fungus bed, this constitutes a natural medium for the growth and development of chanterelles. Termite excreta contain amino acids and soluble nitrogen compounds, and provide a good source of nitrogen for the fir fungus, which is conducive to the growth of the fir fungus mycelium, according to He Xinsheng (1995) observed: ant nests are built in the underground 20-100 cm, the volume ranges from 0.1-10 meters; there is a central large nest and scattered satellite nests, between the ants are interconnected with the ant pathway, the ants in the ants will be in the nest for a long period of time to a few years to a few decades of breeding. The colony will reproduce in the nest for a long time, from several years to several decades.

2. Temperature.

Chicken longitudinal fungus growth in the termite nest, the temperature is stable at 22 ℃ Shi 30C, the lowest 15 ℃, the highest not more than 28 ℃, almost constant temperature. Stable temperature is conducive to the growth of mycelium and the incubation and reproduction of termites. From the artificial domestication test observation: chicken fir fungus holding the child germination temperature range is 12 ℃ a 24 ℃; mycelium growth of the optimal temperature of 16 ℃ -20 ℃, at 18 ℃ from the inoculation to the full tube only 10 days, mycelium grows up to 0.73 cm per day; the formation of the entity of the temperature of 2 5 ℃ -35 ℃ can be the optimal temperature of 25 ℃ -30 ℃, the daily temperature difference of 5 ℃ for the most suitable; higher than 35 ℃, the entity of the child can still develop as usual.

4, humidity.

The stable environmental conditions in the nest are suitable for the growth of mycelium, but not conducive to the formation of the substrate. Therefore, under normal circumstances, the mycelium grows in large quantities in the ant nest and does not form a child entity, need to come to the rainy season, the rain seeps into the soil around the ant nest, at this time the mycelium grows out of the ant nest and produces a child entity on the surface, which indicates the importance of moisture and humidity on the cockle fungus. In artificial cultivation, the moisture content of culture material is 60%-75% can be, with 65% as the best; when the moisture content of culture material is 70%-75%, the formation of fruiting bodies is the most suitable. Chicken fir fungus mycelium growth environment air relative humidity at about 80% is good, the original base formation period air relative humidity can not be less than 80; entity growth stage, the environment air relative humidity of 85% is good, the child entity umbrella needs more than 95% of the air relative humidity.

5, pH.

The pH of the ant nest fungus nursery is relatively stable, often at 4.0-4.5, chemical analysis shows that the king an original prisoner caused by ant A PH 1 E is boy in ten organic acids, such as volatile ant acid, non-volatile aconitic acid, transbutylene diacid, butanedioic acid and so on. The main factor for a more stable pH is the buffering role played by minerals in the nest-building soil and newly transported plant residues. According to He Xinsheng (1995) research chicken fir fungus mycelium growth at pH 4.0-4.5 is the most suitable, and is not conducive to the growth of bacteria and other microorganisms, especially the growth of charcoal hornworm, thus ensuring that the chicken longitudinal bacterium absolute dominance of the position.

6, light.

Spore germination, mycelial growth, protoplast differentiation, the growth and development of the substrate does not necessarily require light. When the substrate is open, it needs a certain amount of diffused light to make it differentiate and develop well.