Tetrodotoxin is that the puffer fish eats the algae containing tetrodotoxin and exists in the body through its own transformation. Originally poisonous puffer fish, if its fry are brought to the pond for artificial breeding, it can lose its toxicity. But if it is fed with bait containing tetrodotoxin, it will be poisoned and produce tetrodotoxin again.

The production of tetrodotoxin is not only closely related to tetrodotoxin feed, but also related to tetrodotoxin acceptance mechanism. In the same sea area, although there are a lot of algae containing tetrodotoxin, some puffer fish eat it and some don't, so there is a difference between poisonous puffer fish and non-toxic puffer fish.