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1. Bovine serum albumin (BSA), also known as the fifth component, is an albumin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.430 kDa and an isoelectric point of 4.7. Bovine serum albumin is widely used in biochemical experiments, for example, as a blocking agent in protein blot; BSA was added to the enzyme digestion reaction buffer to protect the enzyme by increasing the concentration of protein in the solution. Preventing the decomposition and nonspecific adsorption of enzymes can alleviate the denaturation of some enzymes and some unfavorable environmental factors such as heating, surface tension and chemical factors.
Second, the instructions for use
1. Adding BSA to the enzyme digestion reaction buffer can protect the enzyme by increasing the concentration of protein in the solution. Preventing the decomposition and nonspecific adsorption of enzymes can alleviate the denaturation of some enzymes and the denaturation caused by unfavorable environmental factors such as heating, surface tension and chemical factors.
2. It is also commonly used in ELISA. For example, BSA can be used for blocking solutions, sample diluents and enzyme conjugate diluents.
3. Biochemical research, genetic engineering and drug research.