2. Bagging, sterilization and filling. The fermented culture materials were put into bags, each bag was about 1 kg, and the wet weight was about 2 kg. The bag is tightly packed and compacted, and the mouth of the bag is tightly tied with string. Sterilization: put the packaged material bag in a space sterilization bag for 4-5 hours, or put it in an atmospheric soil steamer for 8- 10 hours, cool it until the material bag is not hot, and then take it out and put it in a clean room.
3. Inoculate when the temperature of the inoculation bag drops below 30℃ during construction. After inoculation, they were cultured in a clean room at 15-25℃ in the shade, and the bags were turned over 1 7 days. After 50-60 days of culture, hyphae can grow all over the bag.
4. After the mycelium in the bag is full, strengthen ventilation, control the seedbed temperature at 10-22℃, and keep the soil surface moist. After 8- 10 days of culture, the tumor on the mushroom stick gradually differentiated and expanded into mushroom-like fruiting body. At this time, it is necessary to increase water spraying to increase the relative humidity of the air by 85%-95%. When the mushroom cap extends to 5- 10 cm and the edge of the cap is not fully unfolded, it can be harvested.