In general, after Volvariella volvacea is inoculated 1 day, the inoculated block germinates and grows hyphae, and after 2 ~ 3 days, the hyphae begin to grow into the culture medium, which is called feeding. But occasionally, the inoculated block will not germinate, or it will not be eaten after germination, or it will be contaminated. Therefore, we should pay attention to the inspection of strains when cultivating strains. Seed inspection is an important work of strain culture. Through seed inspection, find problems, analyze reasons and solve them in time.

Volvariella volvacea strains are usually checked twice during culture. The first time: 2 ~ 3 days after inoculation, during which hyphae just germinated and were eaten, and hyphae should not be seen in other parts except the culture surface. If there are contaminated mixed bacteria, the colony of mixed bacteria is the easiest to find, so this is a good time to check the seeds. The focus of the first seed inspection is on the surface of the material, so as to find the pollution point in time, so as to avoid being covered by edible fungus mycelium and creating illusion, so it is necessary to check it carefully. The second time was after the bacteria were all over your face. During this period, the pollution of miscellaneous bacteria is mainly due to the incomplete sterilization caused by the fact that the culture material is not soaked in advance or the bag has tiny sand holes and holes. The main parts of the secondary seed inspection are the side wall, bottom and seal of the bag (bottle).