There are two main cultivation methods in hypsizygus marmoreus: bottle cultivation and bag cultivation. Bottle cultivation method is mainly used for industrial cultivation, which has the advantages of moisture retention and convenient mechanized operation. Bag planting method has large loading capacity, saves labor and is suitable for rural conditions.

(1) formula selection

① 92% cottonseed hull, 5% wheat bran, 2% calcium magnesium phosphate and 0/%gypsum/kloc.

② hardwood sawdust is 74%, wheat bran is 24%, and sugar and gypsum are 65438 0% respectively.

③ Cottonseed hull 38%, sawdust 38%, wheat bran 20%, sugar and gypsum 65438 0% respectively.

④ 40% of corn stalk and corncob powder, 40% of sawdust, 5% of corn flour, 5% of gypsum 1% of sugar 1%.

(2) bagging (bottling) and sterilization

Choose 500 ml cans or 18 cm× 36 cm× 0.03 cm polypropylene and polyethylene plastic bags as cultivation containers. The dry material of each bottle of canned bottles is about 125g, and it is sealed with polypropylene plastic film. Plastic bag cultivation: before bagging, tie one end of the tube film tightly or seal it with one end of the finished bag, and load it from the open end, and compact the material with the back of your fingers while loading. The weight of the packaging bag is 1.5 ~ 1.65 kg, and the length is about 22 cm. The surface is smooth and the tightness is even and moderate. In the process of bottling (bagging), the culture material to be packaged should be stirred frequently to make the upper and lower water contents consistent. After packaging, it is sterilized by high pressure or normal pressure in a fashion pot. In the process of loading and sterilization, handle it with care. If necessary, put a newspaper between the discharged bottle layer and the bottom of the bag pile to avoid damaging the film.

(3) Inoculation and spawning

The fruiting body of hypsizygus marmoreus has the habit of differentiating mushrooms on the strain layer first, so it is required to use enough seeds when inoculating, and keep a certain strain coverage area and surface area. Before inoculation, the strain is crushed to the size of peanut kernel and inoculated on the material surface to make it naturally bulge, which not only increases the mushroom production area, but also facilitates the natural arrangement of fruiting bodies, which not only has high yield, but also has good uniformity. Bag culture can be inoculated at one or both ends.

According to the requirements of mycelium growth on environmental conditions, the inoculated fungus bags can be placed indoors at a temperature of 20 ~ 25℃ and an air humidity of less than 70%, ventilated and protected from light, or in an outdoor open space when the climatic conditions are suitable. The size of the pile should be adjusted according to the season and temperature, and it is forbidden to pile up in large quantities to avoid burning bacteria. When bacteria grow outdoors, the air is fresh, and the temperature difference between day and night will make the gas in the bag expand with heat and contract with cold, which is beneficial to the gas exchange inside and outside the bag, but it should be shaded, and if necessary, it should be piled with films or other materials to keep warm and prevent rain. If the plastic bag used for culture is tied too tightly, there will often be bacteriostatic phenomenon in the later stage of fungal growth, that is, the hyphae at the front end of the colony are short, neat and linear, and in severe cases, yellow bacteriostatic lines appear, and the hyphae stop extending forward. At this time, the rope should be loosened properly, or the mycelium should be tied at a distance of about 2 cm from the front end of the mycelium for ventilation, and the ambient temperature should be reduced.

(4) after-ripening culture

Generally, the mycelium can fill the bag in about 50 days, and the color begins to change from white to khaki. Using natural temperature, sowing in spring and autumn, and fruiting every other season, the process of storing well-developed fungus bags in out-of-season is also the process of post-ripening culture. The length of time depends on the influence of temperature, ventilation, pH value and water content of materials, container loading and lighting. If the temperature is high, the ventilation is good, the pH value is high, the water content is low, and there is some light stimulation, the mycelium matures quickly, otherwise it will take a long time. The general rule is: increase the temperature of bacteria appropriately; If the culture environment is too dry, the air humidity can be appropriately increased; Slightly increase ventilation; Appropriate increase of illumination and temperature difference stimulation can improve the after-ripening effect. It usually takes about 50 days, that is, the after-ripening period is completed. But there is a time difference between bottle culture and bag culture, which is mainly caused by the different degree of gas exchange inside and outside different containers.

Over-summer management: After the fungus bags sown in spring are full, they can be moved into dark and ventilated rooms for over-summer. Can be relatively concentrated emissions; You can also set up a shed outdoors and cover the pile of fungus bags with straw to prevent the mushroom buds from differentiating in advance. In summer, the temperature is high, so it is generally unnecessary to manage, and the mycelium can reach physiological maturity smoothly in late August.

Overwintering management: mainly solve the problem of heat preservation, otherwise the mycelium may be immature in the next spring, which will affect the fruiting. In the early stage, a large pile was produced, and the bag pile was covered with plastic film for insulation. Before fruiting 1 month, if the mycelium is still white, it means that its physiological maturity is not enough, so artificial heating should be carried out and the temperature should be controlled at 25 ~ 30℃ to accelerate the maturity of the mycelium.