Please note that the catalytic hydrolysis ability of protease is closely related to its active center or catalytic hydrolysis site. Many factors, such as different amino acid composition and conformation of active center, may affect the catalytic hydrolysis ability of protease. The efficacy of a single protease is not omnipotent, that is, it is impossible for a single protease to decompose all protein at the same time. Here are some introductions about protease, hoping to help you:

An enzyme that hydrolyzes protein peptide bonds. According to the way of hydrolyzing peptide, it can be divided into endopeptidase and exopeptidase. Endopeptidase cuts off the interior of protein molecules, forming moon and peptone with smaller molecular weight. Exopeptidase hydrolyzes peptide bonds one by one from the free amino or carboxyl end of protein molecule, and dissociates amino acids, the former is aminopeptidase and the latter is carboxypeptidase. According to its active center and optimum pH value, proteases can be divided into serine protease, mercaptoprotease, metalloprotease and acid protease. According to the optimum pH value of the reaction, it can be divided into acid protease, neutral protease and alkaline protease. The protease used in industrial production is mainly endopeptidase.

Proteases are widely found in animal viscera, plant stems and leaves, fruits and microorganisms. Microbial protease is mainly produced by mold and bacteria, followed by yeast and actinomycetes.

An enzyme that catalyzes the hydrolysis of protein. There are many kinds, including pepsin, trypsin, cathepsin, papain and subtilisin. Proteases have strict selectivity to reaction substrates. One protease can only act on some peptide bonds in protein molecules, such as those formed by the hydrolysis of basic amino acids catalyzed by trypsin. Proteases are widely distributed, mainly in the digestive tract of humans and animals, and are rich in plants and microorganisms. Due to the limited animal and plant resources, protease preparations are mainly produced by fermentation of microorganisms such as Bacillus subtilis and Aspergillus terrestris.

Protease has been widely used in leather, fur, silk, medicine, food, brewing and other fields. Protease has been widely used in depilation and softening of leather industry, which not only saves time, but also improves labor hygiene conditions. Protease can also be used for degumming silk, tenderizing meat and clarifying wine. Clinically, it can be used as medicine, such as treating dyspepsia with pepsin, bronchitis with acid protease, vasculitis with fear protease, purifying surgical suppurative wounds with trypsin and chymotrypsin, and treating serosal adhesion between thoracic cavities. Enzyme washing powder is a new product in detergent, which contains alkaline protease. It can remove blood stains and protein dirt from clothes, but be careful not to touch the skin when using it, so as not to damage the protein on the skin surface and cause allergic phenomena such as rash and eczema.