Col and colchicine amide were injected intraperitoneally into mice once a day 1 time for 7 days, and the median lethal dose was 4.95mg/kg and 6 1.6mg/kg, respectively. After 3 days of intraperitoneal injection of colchicine 1-2mg/kg, gastrointestinal peristalsis weakened and gastrointestinal congestion and ulceration appeared. When the total amount of intravenous injection in rabbits is 3.9mg/kg, flatulence and renal damage can be seen. When the anti-tumor dose is reached, the weight of spleen can be reduced by about 50%-60%. In addition, a certain amount of Col can inhibit normal cell division, inhibit bone marrow and cause agranulocytosis and aplastic anemia. Colchicine amide is injected intravenously into dogs at a dose of 0.2-0.4 mg/kg. On the fourth day, vomiting, diarrhea, loss of appetite, elevated alanine aminotransferase and bone marrow suppression occurred, but there was no obvious damage to the kidney. All the above symptoms can return to normal after drug withdrawal. Lijiang Shā rotto Katakuri contains a large amount of Col, which can be oxidized into highly toxic oxydicolchine in the body after oral administration, which has a serious stimulating effect on the digestive system and urinary system and an inhibitory effect on the nervous system. Its lethal dose is about 20-30 mg.

The most common side effects of Col occur in rapidly proliferating gastrointestinal epithelial cells, especially in jejunum, which are mainly manifested as nausea, vomiting and abdominal pain. Once the drug is stopped, the incubation period is about several hours or longer. Intravenous injection of Col is also effective, and it takes effect faster, which can completely avoid gastrointestinal side effects. Col acute poisoning can lead to hemorrhagic gastroenteritis, extensive vascular injury, nephrotoxicity, muscle inhibition and descending paralysis of the central nervous system. Cul can produce temporary leukopenia, followed by leukocytosis, sometimes due to a significant increase in basophils. Long-term use of Col also has the risk of agranulocytosis, aplastic anemia, myopathy and alopecia. There are also reports of sperm deficiency.