1, killing microorganisms-disinfection and sterilization;
2, oxidizing and deodorizing various odorous inorganic or organic substances;
3. Oxidation of metabolites, thus inhibiting the metabolic process.
1、? Killing microorganisms-disinfection and sterilization
Although freezing may kill some bacteria, some pathogenic bacteria have great resistance to low temperature, and once the temperature rises, these bacteria will "revive". Especially in the cold room and cooling room, the temperature is suitable for the growth of psychrophilic bacteria, mold and yeast, which will cause great damage to the stored food. In this case, the use of ozone will achieve satisfactory results. Ozone belongs to gas sterilization agent. The bacteriostatic and bactericidal effects of disinfectants are usually the result of the comprehensive effects of physics, chemistry and biology.
The mechanism can be summarized as follows:
A) It acts on the cell membrane, resulting in the increase of the permeability of the cell membrane and the outflow of intracellular substances, which makes the cells lose their vitality;
B) necessary for cell activity? Lose vitality. These? Could it be basal metabolism? Can it also be an important component of synthetic cells? ;
C) Destroy the genetic material in cytoplasm or make it lose its function. It is generally believed that ozone kills viruses by directly destroying their RNA (ribonucleic acid) or DNA (deoxyribonucleic acid). And ozone kills bacteria and mold microorganisms, which is the first effect of ozone on cell membrane? The composition of cell membrane is destroyed, which leads to metabolic disorder and inhibits its growth. Ozone continues to penetrate and destroy the tissue in the membrane until it dies.
The sterilization effect of ozone depends on ozone concentration, microbial species, treatment time, temperature and humidity of warehouse, materials of walls, ceilings and floors, packaging materials and methods, absorbability of stored articles and oxidation reaction.
Ozone has different killing effects on various microorganisms, and ozone has a strong bactericidal effect on pathogenic bacteria and viruses of human beings and animals. Experiments show that ozone can kill Staphylococcus aureus, Bacillus subtilis, Escherichia coli, hepatitis B surface antigen, Salmonella, Vibrio obliquus and so on. Experiments show that ozone has a better killing effect on mold than bacteria, and ozone has a poor killing effect on bacteria at a low concentration (about 0.2mg/m3), because antibodies will be produced after exposure to this concentration for a certain period of time. Ozone has a similar situation for some molds on fruits. Bacillus spores have strong high temperature resistance. For example, spores of Bacillus subtilis can still maintain vitality when heated at 100℃ for 60 minutes. Ozone can also kill these spores after a long period of treatment. The resistance of lactic acid bacteria to ozone is very weak, and it can be killed in water 15 seconds. Killing effect of ozone on yeast. Generally speaking, 0.5ppm ozone treatment in water for 5- 10 minutes, the killing rate is 100%. * * The research on "Inhibition and Killing Effect of Ozone on Mould Spores" was carried out, and the conclusion was that under the condition of ozone concentration of 12ppm, unsintered spores could be completely killed in 3 hours.
The environmental factors that affect the sterilization effect of ozone are mainly temperature and humidity. When the temperature is low and humidity is high, the killing effect is good, especially when the relative humidity is less than 45%. Ozone has little killing effect on microorganisms suspended in the air. When the relative humidity exceeds 60%, the killing effect gradually increases and reaches the maximum at 95%, which is mainly due to the phase? The increase of humidity makes bacteria swell, making them more susceptible to ozone. Cold storage is low temperature and high humidity, which is beneficial to the application of ozone sterilization.
When ozone is used to disinfect the cold storage, the goods in the cold storage should be emptied, cleaned and disinfected on the ground, and cleaned and dried with pads. Press 10? × 10-3g/m3？ Select the ozone generator, that is, its concentration is 6- 10ppm. After reaching this concentration, stop the machine and seal the warehouse for 24-48 hours. When ozone is used to disinfect empty cold storage, the killing rate of bacteria can reach about 90%, and the killing rate of mold can reach about 80%. The effect is equivalent to peracetic acid, stronger than formaldehyde?
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