Fluorescence quantitative PCR was used to detect the amount of SYBR Green doped with double-stranded DNA. When SYBR Green is mixed with double-stranded DNA, it will emit fluorescence. But as long as it is double-stranded, it is mixed.

Probe method is that after the probe is combined with the target sequence, the fluorescent group on the probe leaves the quenching group after the probe is degraded by polymerase, thus emitting fluorescence.

It is not difficult to judge from the principles of the two methods that the fluorescence PCR method is simpler, more convenient and lower in cost. The detection method is more specific.

Extended data:

Fluorescence quantitative PCR (FQPCR; ); Real-time quantitative qPCR (RT-QCPR or QCPR).

1996, American applied biological systems company introduced a new quantitative detection technology. It marks and tracks PCR products with fluorescent dyes or fluorescent labeled specific probes, analyzes the products with corresponding software, and calculates the initial concentration of the sample template to be tested, thus monitoring the reaction in real time.

Fluorescence quantitative PCR_ Baidu Encyclopedia