1, mother species production technology, generally in the preservation period of the large bulbous cap mushroom strains of mycelium in the slow growth or stop production state, so in the production of the mother species for production, must be preserved before the re-strengthening of the strains, in order to be in the state of exuberant growth of the strains of mushrooms access to the mother species or the original species of the culture medium, to promote the rapid growth of mycelium, to obtain the mycelium of the thick and robust, thick and white, the growth of the potential of strong strains.

(1) Medium formula: No. I: PDA (potato 200g, glucose 18g, agar 18g, water 1000m1). No. II: PDA + magnesium sulfate 1.5g + potassium dihydrogen phosphate 3g + peptone 2g, water 1000 ml. No. III: potato 100g, dry miscellaneous wood chips 100g, dextrose 20g, agar 20g, water 1000 ml.

The above formulations of the medium were made into test-tube slant by high-pressure 20-minute sterilization and disinfection, and were used up within 3 days.

(2) Production method: by tissue separation technique, the selected tissue block of large bulbous cap mushroom was connected to the medium of I (or to the preserved species to be rejuvenated). After 10 to 15 days of cultivation at 24 to 28℃, when the mycelium grows to 1:3 - 1:4 in the test tube, pick the dense and thick part of the front end of mycelium extension and transfer it to the medium of No. II or No. III, and use two-point inoculation method to make the mycelium grow all over the surface of the medium rapidly (generally 15 to 20 days) to improve the quality of the mycelium. The mycelium cultured by No.Ⅱ and No.Ⅲ re-strengthening medium shows thick white, thick and strong vitality, so as to facilitate the growth of the original mycelium, shorten the cultivation time of the original species, and improve the rate of finished products.

2, the original seed and production seed production

(1) formula: I: wheat or barley 88%, rice bran or bran 10%, gypsum or calcium carbonate 1%, lime 0.5%, 65% water content (adding rice bran or bran is mainly to make the medium loose, increase the permeability of mycelium towards good). No. II: 25% wheat, 64% straw, 10% wheat bran, 1% gypsum powder, 65% - 70% water content. No. Ⅲ: 42% of miscellaneous wood chips, 42% of miscellaneous wood flowers, rice bran or wheat bran 15%, 0.2% of magnesium sulfate, gypsum or calcium carbonate 1%, water content of 70%.

The above formula in 1.3 - 1.5kg / square meter pressure, disinfection 2 - 2.5 hours, 3 days to use up.

(2) Selection and preparation of raw materials: Practice has proved that: the main raw material of culture medium for the production of original and production species of large bulbous cap mushrooms is the best wheat grain, followed by wood shavings, wood shavings, straw is the worst.

(3) the choice of wheat: should choose deep yellow or dark red skin toughness, soaking is not easy to break the skin of the wheat grain is good, from the water absorption rate of barley is better than wheat.

(4) soaking: soaking lime water soaking and water soaking pre-cooked with lime two methods. The former with 1% lime water soaking, pH 9 to 10, at 25 ℃ wheat soaking 8 to 9 hours, barley soaking 9 to 10 hours, high water temperature in summer should be appropriate to shorten the time. Soaked wheat grains, pinch the wheat grain depression with fingernail, there is a strong elasticity, nail prints quickly disappear as appropriate. The latter with water first soaked grains of wheat, time and on the same. Soaked grains of wheat flooded up with water and rinsed and drained, put into the pot to boil until just flooded up and cooled off, add to the mixture of materials, with 0.5% lime pH 6.5 or so, 65% water content.

(5) bottling and disinfection: choose the volume of 700ml, the inner diameter of the mouth of the bottle is 3cm or so of the light white heat-resistant glass bottles (should not be used with wide-mouth bottles, so as to avoid the invasion of fungi), will be matched with a good mix of culture materials into bottles, each bottle filled with 200 - 300g is appropriate, 1.3 to 1.5kg / square meter pressure disinfection of 2-2.5 hours, do not disinfect the time is too long to avoid Wheat grains are mushy, resulting in decreased permeability and nutrients, affecting mycelial growth.

(4) inoculation and culture: take multi-point inoculation, that is, in the bottle around the culture material according to the appropriate distance inoculation 3 - 4 points, the material surface and then connected to a small piece of mycelium, in the 24-28 ℃ conditions, dark culture 7 - 10 days, mycelium germination and growth to the diameter of 4cm or so of the plaque, and immediately on the mycelium of the artificial stirring, stirring when the inoculation point of mycelium is stirred off the stimulation, and because of the Open the mouth of the bottle (in the ultra-clean table) actually give the mycelium to play the role of oxygen, is conducive to the rapid growth of mycelium, generally the whole process of cultivation takes 25 - 30 days to grow full bottle.

3, the production of species production:

(1) formula, medium production, bottle selection and sterilization and the same as the original species.

(2) inoculation: the original bottle will be full of bottle inoculation to the surface of the production medium, as far as possible to spread the surface of the material, so as to avoid contamination of stray bacteria, each bottle of the original species to receive no more than 30 bottles of production species, cultured at 24-28 ℃ under the conditions of the mycelium sprouting 7-10 days to grow to the white, dense and thick, the mycelium of the artificial mixing, stirring the mycelium to stimulate its rapid growth, generally 20-25 days full of growth The whole bottle.