(1) Cultivation season: Agaricus bisporus is a mesophilic edible fungus, which can be cultivated all year round. The optimum temperature for mycelium growth is 22℃-25℃, and the optimum temperature for mushroom bud formation and growth is 14℃- 16℃.

(2) Selection of culture medium: PDA culture medium is used for mother seed, and wheat grain culture medium is better for original seed and cultivated seed, and it is made by routine method. The age of bacteria should not be too long.

(3) The formula of cultivation material: wheat straw 62%, dry cow dung 3 1%, rapeseed cake 3%, urea 0.3%, gypsum 1.7%, calcium superphosphate 0.30% and lime 1.7%.

(4) Stacking fermentation of culture materials: the pile width is 2.3-2.5m, the height is1.5-1.8m, and the length is generally not less than 5m. After the culture material is piled for 6-7 days, when the temperature reaches 70℃-75℃, it is turned over for the first time and gypsum is added; Turning the pile for the second time after 5-6 days, and adding lime; Another 4? After 5 days, turn the pile for the third time and add calcium superphosphate. After 3-4 days, the material is moved to the sterilized mushroom house while it is hot, and the second fermentation is carried out. The water content of fermented material is 62%, and the pH value is 7? 7.5。

(5) Manufacture of cultivation beds: make cultivation beds with the width of1.4m in the mushroom growing place, and leave 20cm in the beds as walkways. Put the fermented culture material into a cultivation bed with a thickness of 20 cm.

(6) Sowing and post-sowing management: The method of withdrawing sowing is often adopted. First, two-thirds of the strains are scattered on the material surface, and the material is gently stirred by hand to evenly mix the strains with the upper half of the culture material, and then the remaining one-third of the strains are scattered on the material surface, and the culture material is gently patted flat. Close the doors and windows 3 days after sowing, and gradually increase the ventilation volume in the morning and evening after 3 days. In 7- 10 days, hyphae basically grow on the material surface, open the vent and ventilate day and night.

(7) Covering the soil: firstly, covering the coarse soil, spreading the large grains evenly to cover the material surface, with a thickness of 3cm, and covering the coarse soil with a thickness of 0.5- 1 cm when the hyphae penetrate into the coarse soil and grow to half the height of the coarse soil.

(8) Autumn mushroom management: the humidity of mushroom house is kept at 80%-90%, the temperature is kept at 20℃-22℃, and proper ventilation is carried out.

(9) Overwintering management: The temperature is not lower than 0℃, and water is sprayed every 10- 15 days/time.

(10) spring mushroom management: keep the temperature of mushroom house 14℃_ 18℃, and spray 0. 1%-02% urea solution/kloc-0 according to the soil filling on the bed surface 1 cm when the mushroom buds are as big as soybeans.

(1 1) harvesting: harvesting in time when the diameter of mushroom cap is 2.5-4cm. When harvesting, hold the mushroom cover by hand and gently rotate it to harvest, so as not to damage the culture bed as much as possible.