Temperature control is the key to cultivate Pleurotus ostreatus in greenhouse, which is directly related to the quality and yield of Pleurotus ostreatus. Temperature control is beneficial to the growth of culture medium, the growth and senescence of hyphae, the size and meat quality of fruiting bodies, the yield and the management of pests and diseases. Pleurotus ostreatus is a kind of low-temperature mushroom, and the temperature of mycelium growth should be between 7 ~ 30℃, and the most suitable temperature is 24 ~ 27℃. The maximum temperature of spawning in Pleurotus ostreatus culture medium should not exceed 30℃, otherwise the mycelium will easily age prematurely and affect the yield. The temperature of fruiting body formation is 7 ~ 22℃, and the optimum temperature is 15 ~ 18℃. Pleurotus ostreatus is an edible fungus with variable temperature. When fruiting, widening the temperature difference between day and night can promote the differentiation, formation and growth of fruiting bodies and effectively increase the yield of Pleurotus ostreatus. Pleurotus ostreatus can tolerate growth at 0 ~ 7℃ and has strong cold resistance. It grows slowly at-1 ~ 5℃, and the mycelium will not die at -30℃.

During the cultivation of Pleurotus ostreatus, the temperature is controlled between 15 ~ 18℃, which is the most suitable temperature for growth. Pleurotus ostreatus has good quality and high yield, reaching the maximum optimization degree. Pleurotus ostreatus is mostly cultivated in greenhouses, greenhouses and other facilities, so indoor temperature and humidity adjustment is very important, because it cannot rely on external adjustment. Most hyphae can grow at 5-35 degrees, but the optimum temperature is 20-30 degrees. For example, the optimum growth temperature of Pleurotus eryngii mycelium is 24-27 degrees, and the fruiting body is 13- 18 degrees. Pleurotus ostreatus likes humidity, and sometimes farmers will put it next to the sink to grow it. The growth humidity of mycelium should be 55%-60% in cottonseed hull culture medium and 70% in wheat straw, even if the environmental humidity is high, 50% will not happen.

The growth of fruiting bodies requires high environmental humidity, generally 80%-85%, and the humidity is between 70% and 95%. Too low will not produce mushrooms, too high will grow slowly. In winter, the temperature is low, the evaporation in the shed is generally small, and the respiration of mycelium decreases at low temperature, and the water consumption also decreases accordingly. Moreover, in order to ensure the temperature, the number and time of uncovering the film are also reduced, which doomed the relative stability and balance of water in the shed. Therefore, when planting Pleurotus ostreatus in winter, water spraying should be appropriate, not too much. Ventilation is a measure to ensure the oxygen content in the planting room, but too much ventilation or too long time will easily lead to the temperature drop in the planting room. Therefore, when planting Pleurotus ostreatus in winter, the ventilation should be frequent and not large.