The concentration should be appropriate, not too high.

Bovine serum albumin (BSA) contains 607 amino acid residues, molecular weight is 66.446 kDa, isoelectric point is 4.7. BSA is widely used in biochemical experiments.

In the PCR system, it helps the stability and activity of Taq enzyme, and can improve the efficiency of PCR. 10 grams of it can be dissolved in 100 ml of water, or dissolved in PBS, depending on the use.

Dispense into small sticks. Can be stored in a thermostat at -20 or 30 degrees Celsius without preservatives, or at -4 degrees Celsius with preservatives (e.g., 0.1% sodium azide). It can generally be stored for several months without deterioration. Preservatives may have an effect on the effectiveness of bovine serum albumin and should be used with caution.

Expanded Information:

Bovine Serum Albumin Component V is best used as it is formulated. solution can cause your results to be high even after being stored at -20 degrees for a while.

Simple proteins in bovine serum, the main component of blood (38g/1000ml), have a molecular weight of 68kD. isoelectric point of 4.8. nitrogen content of 16% and sugar content of 0.08%. It contains only hexose and hexosamine, and only 0.2% lipid. Albumin consists of 581 amino acid residues, of which 35 cysteines form 17 disulfide bonds, and there is a free sulfhydryl group at the 34th position of the peptide chain.

Baidu Encyclopedia - Bovine Serum Albumin