bǔ shèn gù chǐ wán
2 English referencebushen guchi pills [Nomenclature of Traditional Chinese Medicine (2004)], a Chinese medical terminology review and approval committee. Chinese Medicine Nomenclature (2004)]
bushen guchi wan [Nomenclature Review Committee of Chinese Medicine. It has the effects of tonifying the kidney and fixing the teeth, promoting blood circulation and detoxification. It is used to treat tooth weakness caused by kidney deficiency and fire exuberance, weakness in chewing, loosening and displacement, gingival swelling and epistaxis; chronic periodontitis with the above symptoms. Zedoary, cinnamon
4.3 MethodThe above eighteen flavors, crushed into fine powder, sieve, mix. Another 47g of salt, dissolved with appropriate amount of water, filtered. Take the above fine powder, salt water pills, dry, film coating, that is obtained.
4.4 CharacterThis product is a film-coated water pill, brown after removing the coating; taste salty, slightly bitter, pungent.
4.5 Identification(1) take the product, placed under the microscope: thin-walled tissue gray-brown to black-brown, the cells are wrinkled, containing brown nuclei (yellow). Seed skin epidermal stone cells yellowish brown, surface view of the class polygonal, thick wall, pore groove dense, cavity containing dark brown material (wine schizandra). Fibers in bundles or scattered, thick wall, longitudinal cracks on the surface, the ends broken into broom-like or more flat truncation (Astragalus). The pollen grains are orbicular, 24-34 μm in diameter, with spines on the outer wall, 3-5 μm in length, and with three germination holes (Chrysanthemum officinale). Seed coat stone cell surface view irregular polygonal, thick wall, undulate curved, clear laminae (Lycium barbarum).
(2) take 10g of this product, crushed, add 30ml of trichloromethane, heat and reflux for 30 minutes, filtration, filtrate extracted with 40ml of water and shaking, discard the aqueous solution, trichloromethane solution evaporated, the residue with 2ml of methanol to make soluble, as a test solution. Take 1g of Fructus Schisandrae chinensis control herb, and make control herb solution by the same method. Then take Schisandra chinensis control, add methanol to make a solution containing 1mg per 1ml; take tanshinone IIA control, add methanol to make a solution containing 2mg per 1ml, as the control solution. According to the thin-layer chromatography (2010 Pharmacopoeia, Appendix VI B), absorb 8μl of the test solution, 4μl of the control solution and 4μl of each of the above two control solutions, respectively, spot on the same silica gel GF254 thin-layer plate, using toluene-ethyl acetate-formic acid (9:1:0.2) as the unfolding agent, unfold, remove, dry, respectively, in daylight and then in the sunlight, and then in the dark. Drying, respectively, in daylight and ultraviolet light (254nm) under the examination. In the chromatogram of the test article, in the corresponding position with the chromatogram of tanshinone IIA control article. In the chromatogram of the test article, in the corresponding position with the chromatogram of tanshinone IIA control article, the same red spot was shown under sunlight; in the corresponding position with the chromatogram of the control herb and the chromatogram of Schisandra chinensis control article, the main spot of the same color and the spot of the same color were shown under ultraviolet light.
(3) take 20g of this product, crushed, add 50ml of methanol, ultrasonic treatment for 20 minutes, filtration, filtrate in a water bath evaporation, residue add ethyl acetate 1ml to dissolve, as a test solution. Take 0.5g of the control herb of Leaky Grouse, and make a solution of the control herb according to the same method, according to the test of thin-layer chromatography (2010 version of Pharmacopoeia, Appendix VI B), absorb 8μl of each of the above two solutions, and put them on the same silica gel G thin-layer plate respectively, use petroleum ether (60-90℃) - ethyl acetate (10:1) as the unfolding agent, unfold it, take it out, let it dry, and check under the ultraviolet lamp (365nm). (365 nm). In the chromatogram of the test article, in the corresponding position with the chromatogram of the control herb, the fluorescent spots of the same color.
(4) take 20g of this product, crushed, add 50ml of methanol, heating reflux for 1 hour, filtration, filtrate evaporation, residue with 15ml of water to make dissolved, water saturated n-butanol shaking extraction 3 times, 20ml each time, combined with the n-butanol solution, washed with ammonia solution 2 times, 30ml each time, and then washed with water saturated with n-butanol twice, 30ml each time, n-butanol recovery solvent solution to about 2ml, add neutralized n-butanol. To about 2ml, add neutral alumina (100-120 mesh) 3g, stir, evaporate, add in neutral alumina column (100-120 mesh, 2g, inner diameter of 10-15mm), elute with 40% methanol 100ml, collect the eluent, evaporate, the residue add methanol 1ml to make dissolved, as a test solution. Another take astragalus control herb 1g, add methanol 30ml, the same method into the control herb solution. Then take astragalus methyl glycoside control, add methanol to make a solution containing 1mg per 1ml, as the control solution. According to the thin layer chromatography (2010 Pharmacopoeia, Appendix VI B), absorb 10μl of the test solution, the control solution and the control solution of 3μl, respectively, on the same silica gel G thin layer plate, with trichloromethane-methanol-water (13:7:2) 10 ℃ below the lower solution as the unfolding agent, unfolding, remove, dry, sprayed with water, then sprayed with water, then sprayed with water. Remove, dry, spray with 10% sulfuric acid ethanol solution, and heat at 105 ℃ until the spots show clear color. In the chromatogram of the test article, in the corresponding position with the chromatogram of the control herb and the chromatogram of the control article, the spot of the same color.
(5) take 20g of this product, crushed, add 30ml of methanol, heat reflux for 1 hour, filtration, filtrate evaporation, residue plus 25% sulfuric acid solution 30ml to dissolve, heat reflux for 2 hours, cool, with ether shaking extraction 2 times, each time 30 ml, combined with ether extracts, evaporation, residue plus 2ml of ethanol to dissolve as a solution for the test product. Take 1g of the control herb of hyssop, and make the solution of control herb by the same method. According to thin-layer chromatography (2010 version of Pharmacopoeia, Appendix VI B) test, absorb the above two solutions of 10μl, respectively, point in the same silica gel G thin-layer plate, with toluene - ethyl acetate - formic acid (4: 1: 0.5) of the upper layer of the solution as an unfolding agent, unfolding, take out, drying, sprayed with 10% sulfuric acid ethanol solution, heating Until the spots showed clear color. In the chromatogram of the test material, the corresponding position with the chromatogram of the control herb, the same color spots.
4.6 InspectionIt should comply with the provisions under the pills (2010 version of the Pharmacopoeia, Appendix I A).
4.7 Determination of contentDetermined by high performance liquid chromatography (2010 Pharmacopoeia, Appendix VI D).
4.7.1 Chromatographic conditions and system suitability testOctadecylsilane-bonded silica gel was used as filler; methanol-acetonitrile-formic acid-water (30:10:1:59) was used as the mobile phase; the detection wavelength was 286 nm; the theoretical plate number should be not less than 2000 according to the calculation of the peaks of salvia B.
4.7.2 The chromatographic conditions were as follows
4.7.2 Preparation of control solutionTake the appropriate amount of salvianolic acid B control, precision weighing, add 50% methanol to make a solution containing 50 μg per 1 ml, that is, obtained.
4.7.3 Preparation of test solutionTake appropriate amount of this product. Crushed, through the fourth sieve, take about 2g, precision weighing, placed in a stoppered conical flask, precision addition of 50% methanol 50ml, weighing, heating reflux for 3 hours, cooled, and then weighing, methanol to make up for the loss of weight, shaking, filtration, take the filtrate, that is, obtained.
4.7.4 DeterminationPrecisely aspirate 10μl each of the control solution and test solution, inject into the liquid chromatograph, determination, that is, obtained.
Each 1g of this product contains not less than 1.5mg of Salvia divinorum in terms of Danshenolic acid B (C36H30H16).
4.8 Functions and IndicationsNourishing the kidneys and fixing the teeth, invigorating the blood and removing toxins. It is used in treating soreness and softness of teeth, weakness of mastication, loosening and displacement, gingival swelling and epistaxis caused by kidney deficiency and fire exuberance; chronic periodontitis with the above symptoms.
4.9 Dosage and AdministrationOral. 4g once, twice a day.
4.10 Specifications1g per 30 pills
4.11 StorageSealed.
4.12 VersionChinese People's Pharmacopoeia 2010 Edition
5. 5.1 Name of the medicineBushen Guchi Wan
5.2 Pinyin of the medicineBushen Guchi Wan
5.3 Dosage form1g for each 30 pills
5.4 CharacterBushen Guchi Wan is a film-coated aqueous pill, with the capsule removed, it can be taken twice a day, once for each 30 pills, twice a day for each 30 pills, twice a day, twice a day, twice a day. Film-coated water pill, brownish brown after removing the coating; taste salty, slightly bitter, pungent.
5.6 Functions and Indications of Nourishing Kidney and Fixing Teeth PillNourishing kidney and fixing teeth, promoting blood circulation and detoxification. It is used in treating soreness and weakness of teeth, weakness in chewing, loosening and displacement, gingival swelling and epistaxis caused by deficiency of kidney and fire; chronic periodontitis with the above symptoms.
5.7 Usage and dosage of Tonifying Kidney and Fixing Teeth PillOral. 4g once, twice a day.
5.8 Precautions1. Avoid smoking, alcohol and spicy, greasy food. Do not eat too hard food.
2. People with hypertension, heart disease, liver disease, diabetes, kidney disease and other serious chronic diseases should take it under the guidance of a physician.
3. Pregnant women, the elderly and infirm should take under the guidance of a physician.
4. It is best to take the drug with stomatological treatment.
5. If the symptoms are not relieved after 7 days of taking the medicine, you should go to the hospital.
6. Allergy to Kidney Supplement Pills is prohibited, and allergy sufferers should be cautious.
7. Kidney Repairing Pills are prohibited when there is a change in the character of the pills.
8. Please keep Kidney Repair Pills out of the reach of children.
9. If you are using other medicines, consult your physician or pharmacist before using Kidney Supplement Pills.
5.9 Drug interactionsDrug interactions may occur if used with other drugs, consult your physician or pharmacist for details.
5.10 Remarks