2, the preparation before making strains. Prepare a sterilized pool, will cut off the dried branches without mold placed in the pool. Height accounted for about 60% of the pool, and then covered, to prevent the branches from floating, and then, injected with water. About 130% of the branches. Continue to soak for about 40 hours and check. Check to see if they have soaked through. Remove the cover and add a small amount of quicklime to the soaked branches. Stirring is carried out and the ph value takes on an alkaline character. Choose polypropylene seed bags, according to their own needs, generally use the specifications of 15cm x 35cm special bags. Drain the water from the branches, bagging, about one hundred per bag. Seal the mouth can not be sealed too tight. In order to prevent the steam gas expansion to break the plastic bag. Well-filled paper strips of the ground, put in the autoclave, autoclave sterilization. High-pressure cooker, maintained at two air pressure, about 3 hours, room temperature needs to be more than ten hours of high-temperature sterilization. Then cool to room temperature standby.
3, prepare the inoculation box for inoculation. Generally, the fall cultivation of strains in the summer for seed production. Spring cultivation of strains in winter seeding. General use of aerosol sterilization 40 minutes after the operation can be inoculated. Will be made of the original seed block, soybean grain size block for inoculation. Put the inoculated bags on the fungus rack in the constant temperature room. The temperature should be maintained at 24-27 degrees. The temperature difference should not be too large, so as not to affect the growth of mycelium. In the process of germination, timely check whether there is contaminated by stray bacteria, eliminating the treatment of contaminated by stray bacteria, generally, after 6-7 weeks, you can send a full bag of fungi.