Keratin is the main component of rhinoceros horn. In addition, it also contains other protein, peptides and free amino acids, guanidine derivatives, sterols and so on. Among the amino acids of rhino horn protein, cystine accounts for 8.7%, and there are three basic amino acids: arginine, lysine and arginine. Its molecular number ratio is 1: 5: 12, so it is similar to wool and horn, and mainly belongs to true keratin. In medical treatment, cheap buffalo horn is often used instead of rhinoceros horn, which promotes the study of horns. For the comparison of the composition of the two corners, please refer to the buffalo horn strip. Simply put, both horns contain cholesterol, but rhinoceros horn also contains trace amounts of allosterols and amino acids, which are components of basic peptides. Aspartic acid is found in rhinoceros horn, but not in buffalo horn. Rhinoceros horn contains less guanidine than buffalo horn. The decoction of rhinoceros horn was electrophoresed on paper, and there were two ninhydrin positive spots on the anode side which were slightly slower than cysteine. After hydrolysis, there is cysteine. We also found that 1 acidic amino acid was slightly slower than cysteine. 2 mg/g amino acids can be obtained by extracting rhinoceros horn with hot water, which contains about 20 kinds of amino acids such as serine and glycine. The decoction also contains ethanolamine.