Identification of Polygonum cuspidatum leaves
Take 5g of this product powder, add 50ml of mixed solution of methanol and 25% hydrochloric acid (4: 1), heat and reflux 1 hour, filter, and take the filtrate as the test solution. Another quercetin reference substance was added with ethanol to prepare a solution containing 0.5 mg per kloc-0/ml as the reference substance solution. According to the test of thin-layer chromatography (China Pharmacopoeia Appendix 1), absorb 65,438+0μ l of the above two solutions, respectively spot them on the same silica gel H plate, use sodium carboxymethyl cellulose as adhesive and toluene-acetic acid-ethyl formate (5: 2: 65,438+0) as developing agent, spread, take out, dry and spray 65. In the chromatogram of the test sample, fluorescent spots with the same color appear in the position corresponding to the chromatogram of the control sample. Or take a photo with the product.